View source for 6f88


==Crystal structure of cytochrome P450 CYP260A1 (S276N) bound with progesterone==
<StructureSection load='6f88' size='340' side='right' caption='[[6f88]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6f88]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Polyangium_cellulosum_(strain_so_ce56) Polyangium cellulosum (strain so ce56)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F88 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=STR:PROGESTERONE'>STR</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sce1588 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=448385 Polyangium cellulosum (strain So ce56)])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f88 OCA], [http://pdbe.org/6f88 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f88 RCSB], [http://www.ebi.ac.uk/pdbsum/6f88 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f88 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The production of regio- and stereoselectively hydroxylated steroids is of high pharmaceutical interest and can be achieved by cytochrome P450-based biocatalysts. CYP260A1 from Sorangium cellulosum strain So ce56 catalyzes hydroxylation of C19 or C21 steroids at the very unique 1alpha-position. However, the conversion of progesterone (PROG) by CYP260A1 is very unselective. In order to improve its selectivity we applied a semirational protein engineering approach, resulting in two different, highly regio- and stereoselective mutants by replacing a single serine residue (S276) of the substrate recognition site 5 with an asparagine or isoleucine. The S276N mutant converted PROG predominantly into 1alpha-hydroxy-PROG, while the S276I mutant led to 17alpha-hydroxy-PROG. We solved the high-resolution crystal structures of the PROG-bound S276N and S276I mutants, which revealed two different binding modes of PROG in the active site. The orientations were consistent with the exclusive 1alpha- (pro-1alpha binding mode) and 17alpha-hydroxylation (pro-17alpha-binding mode) of S276N and S276I, respectively. We observed that water-mediated hydrogen bonds contribute to the stabilization of the polar C3 and C17 substituents of PROG. Both binding modes of PROG may be stabilized in the wild-type enzyme. The change in regioselectivity is mainly driven by destabilizing the alternative binding mode due to steric hindrance and hydrogen bond disruption, caused by the mutations of Ser276. Thus, for the first time, the change in the selectivity of cytochrome P450-mediated steroid hydroxylation created by rational mutagenesis can be explained by the obtained 3D structures of the substrate-bound mutants, providing the basis for further experiments to engineer the biocatalyst toward novel steroid hydroxylation positions.

Structure-Based Engineering of Steroidogenic CYP260A1 for Stereo- and Regioselective Hydroxylation of Progesterone.,Khatri Y, Jozwik IK, Ringle M, Ionescu IA, Litzenburger M, Hutter MC, Thunnissen AWH, Bernhardt R ACS Chem Biol. 2018 Mar 13. doi: 10.1021/acschembio.8b00026. PMID:29509407<ref>PMID:29509407</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6f88" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Jozwik, I K]]
[[Category: Thunnissen, A M.W H]]
[[Category: Bacterial protein]]
[[Category: Biocatalysis]]
[[Category: Cytochrome p-450 enzyme system]]
[[Category: Cytochrome p450]]
[[Category: Heme]]
[[Category: Histidine]]
[[Category: Hydroxylation]]
[[Category: Oxidation-reduction]]
[[Category: Oxidoreductase]]
[[Category: Progesterone]]
[[Category: Sorangium cellulosum]]