View source for 6fbk


==Crystal structure of the human WNK2 CCT-like 1 domain in complex with a WNK1 RFXV peptide==
<StructureSection load='6fbk' size='340' side='right' caption='[[6fbk]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6fbk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FBK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FBK FirstGlance]. <br>
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fbk OCA], [http://pdbe.org/6fbk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fbk RCSB], [http://www.ebi.ac.uk/pdbsum/6fbk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fbk ProSAT]</span></td></tr>
</table>
== Disease ==
[[http://www.uniprot.org/uniprot/WNK1_HUMAN WNK1_HUMAN]] Hereditary sensory and autonomic neuropathy type 2;Pseudohypoaldosteronism type 2C. The disease is caused by mutations affecting the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry. 
== Function ==
[[http://www.uniprot.org/uniprot/WNK2_HUMAN WNK2_HUMAN]] Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.<ref>PMID:17667937</ref> <ref>PMID:18593598</ref> <ref>PMID:21733846</ref>  [[http://www.uniprot.org/uniprot/WNK1_HUMAN WNK1_HUMAN]] Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L.<ref>PMID:10660600</ref> <ref>PMID:15060842</ref>  
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Arrowsmith, C H]]
[[Category: Bountra, C]]
[[Category: Bufton, J C]]
[[Category: Bullock, A N]]
[[Category: Burgess-Brown, N A]]
[[Category: Edwards, A M]]
[[Category: Pinkas, D M]]
[[Category: Ai domain]]
[[Category: Cct domain]]
[[Category: Peptide binding protein]]
[[Category: Rfxv motif binding]]
[[Category: Wnk2]]