View source for 6fn3


==X-ray structure of animal-like Cryptochrome from Chlamydomonas reinhardtii==
<StructureSection load='6fn3' size='340' side='right'caption='[[6fn3]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6fn3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FN3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FN3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fn3 OCA], [https://pdbe.org/6fn3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fn3 RCSB], [https://www.ebi.ac.uk/pdbsum/6fn3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fn3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8J8W0_CHLRE A8J8W0_CHLRE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Photolyases and cryptochromes form an almost ubiquitous family of blue light photoreceptors involved in the repair and maintenance of DNA integrity or regulatory control. We found that one cryptochrome from the green alga Chlamydomonas reinhardtii (CraCRY) is capable of both, control of transcript levels and the sexual cycle of the alga in a positive (germination) and negative manner (mating ability), as well as catalyzing the repair of UV-DNA lesions. Its 1.6 A crystal structure shows besides the FAD chromophore an aromatic tetrad that is indispensable in animal-like type I cryptochromes for light-driven change of their signaling-active redox state and formation of a stable radical pair. Given CraCRY's catalytic activity as (6-4) photolyase in vivo and in vitro, we present the first co-crystal structure of a cryptochrome with duplex DNA comprising a (6-4) pyrimidine-pyrimidone lesion. This 2.9 A structure reveals a distinct conformation for the catalytic histidine His1, H357, that challenges previous models of a single-photon driven (6-4) photolyase mechanism.

Structure of the bifunctional cryptochrome aCRY from Chlamydomonas reinhardtii.,Franz S, Ignatz E, Wenzel S, Zielosko H, Putu EPGN, Maestre-Reyna M, Tsai MD, Yamamoto J, Mittag M, Essen LO Nucleic Acids Res. 2018 Jul 19. pii: 5055825. doi: 10.1093/nar/gky621. PMID:30032195<ref>PMID:30032195</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6fn3" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cryptochrome 3D structures|Cryptochrome 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chlamydomonas reinhardtii]]
[[Category: Large Structures]]
[[Category: Essen L-O]]
[[Category: Franz S]]
[[Category: Ignatz E]]
[[Category: Mittag M]]
[[Category: Wenzel S]]
[[Category: Yamamoto J]]
[[Category: Zielosko H]]