View source for 6fsx


==The hit-and-return system enables efficient time-resolved serial synchrotron crystallography==
<StructureSection load='6fsx' size='340' side='right'caption='[[6fsx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6fsx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodopseudomonas_palustris_CGA009 Rhodopseudomonas palustris CGA009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FSX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fsx OCA], [https://pdbe.org/6fsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fsx RCSB], [https://www.ebi.ac.uk/pdbsum/6fsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fsx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEHA_RHOPA DEHA_RHOPA] Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has lower activity towards bromoacetate and chloroacetate.<ref>PMID:21510690</ref> <ref>PMID:21510690</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.,Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD Nat Methods. 2018 Nov;15(11):901-904. doi: 10.1038/s41592-018-0180-2. Epub 2018, Oct 30. PMID:30377366<ref>PMID:30377366</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6fsx" style="background-color:#fffaf0;"></div>

==See Also==
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodopseudomonas palustris CGA009]]
[[Category: De Gasparo R]]
[[Category: Diederich F]]
[[Category: Jha A]]
[[Category: Mehrabi P]]
[[Category: Miller D]]
[[Category: Mueller-werkmeiser H]]
[[Category: Pai E]]
[[Category: Persch E]]
[[Category: Schulz EC]]
[[Category: Stuart W]]
[[Category: Tellkamp F]]