View source for 6fy6


==Concerted dynamics of metallo-base pairs in an A/B-form helical transition (major species)==
<StructureSection load='6fy6' size='340' side='right'caption='[[6fy6]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6fy6]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FY6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6fy7|6fy7]], [[6rls|6rls]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fy6 OCA], [http://pdbe.org/6fy6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fy6 RCSB], [http://www.ebi.ac.uk/pdbsum/6fy6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fy6 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Metal-mediated base pairs expand the repertoire of nucleic acid structures and dynamics. Here we report solution structures and dynamics of duplex DNA containing two all-natural C-Hg(II)-T metallo base pairs separated by six canonical base pairs. NMR experiments reveal a 3:1 ratio of well-resolved structures in dynamic equilibrium. The major species contains two (N3)T-Hg(II)-(N3)C base pairs in a predominantly B-form helix. The minor species contains (N3)T-Hg(II)-(N4)C base pairs and greater A-form characteristics. Ten-fold different (1)J coupling constants ((15)N,(199)Hg) are observed for (N3)C-Hg(II) (114 Hz) versus (N4)C-Hg(II) (1052 Hz) connectivities, reflecting differences in cytosine ionization and metal-bonding strengths. Dynamic interconversion between the two types of C-Hg(II)-T base pairs are coupled to a global conformational exchange between the helices. These observations inspired the design of a repetitive DNA sequence capable of undergoing a global B-to-A-form helical transition upon adding Hg(II), demonstrating that C-Hg(II)-T has unique switching potential in DNA-based materials and devices.

Concerted dynamics of metallo-base pairs in an A/B-form helical transition.,Schmidt OP, Jurt S, Johannsen S, Karimi A, Sigel RKO, Luedtke NW Nat Commun. 2019 Oct 23;10(1):4818. doi: 10.1038/s41467-019-12440-x. PMID:31645548<ref>PMID:31645548</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6fy6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Johannsen, S]]
[[Category: Jurt, S]]
[[Category: Karimi, A]]
[[Category: Luedtke, N W]]
[[Category: Schmidt, O P]]
[[Category: Sigel, R K.O]]
[[Category: A-form helix]]
[[Category: B-form helix]]
[[Category: Dna]]
[[Category: Metallated duplex dna]]