View source for 6gbv


==A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - M49T mutant==
<StructureSection load='6gbv' size='340' side='right' caption='[[6gbv]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gbv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dinsh Dinsh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GBV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dshi_2006 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=398580 DINSH])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gbv OCA], [http://pdbe.org/6gbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gbv RCSB], [http://www.ebi.ac.uk/pdbsum/6gbv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gbv ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Light, oxygen, voltage (LOV) proteins, a ubiquitously distributed class of photoreceptors, regulate a wide variety of light-dependent physiological responses. Due to their modular architecture, LOV domains, i.e. the sensory domains of LOV photoreceptors, have been widely used for the construction of optogenetic tools. We recently described the structure and function of a short LOV protein (DsLOV) from the marine phototropic bacterium Dinoroseobacter shibae, for which, in contrast to other LOV photoreceptors, the dark state represents the physiologically relevant signaling state. Among bacterial LOV photoreceptors, DsLOV possesses an exceptionally fast dark recovery, corroborating its function as "dark" sensor. To address the mechanistic basis of this unusual characteristic, we carried out a comprehensive mutational, kinetic, thermodynamic and structural characterization of DsLOV. The mechanistic basis of the fast dark recovery of the protein was revealed by mutation of the previously noted uncommon residue substitution at position 49 found in DsLOV. The substitution of M49 by different residues that are naturally conserved in LOV domains tuned the dark recovery time of DsLOV over three orders of magnitude, without grossly affecting its overall structure or light-dependent structural change observed for the wild-type protein. Our study thus provides a striking example of how nature can achieve LOV photocycle tuning by subtle structural alterations in the LOV domain active site, highlighting the easy evolutionary adaptability of the light sensory function. At the same time, our data provides guidance for the mutational photocycle tuning of LOV domains, with relevance for the growing field of optogenetics.

Mechanistic basis of the fast dark recovery of the short LOV protein DsLOV from Dinoroseobacter shibae.,Fettweiss T, Rollen K, Granzin J, Reiners O, Endres S, Drepper T, Willbold D, Jaeger KE, Batra-Safferling R, Krauss U Biochemistry. 2018 Jul 10. doi: 10.1021/acs.biochem.8b00645. PMID:29989797<ref>PMID:29989797</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6gbv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dinsh]]
[[Category: Batra-Safferling, R]]
[[Category: Granzin, J]]
[[Category: Roellen, K]]
[[Category: Light-oxygen-voltage]]
[[Category: Lov]]
[[Category: Pa]]
[[Category: Signaling protein]]