View source for 6gdg


==Cryo-EM structure of the adenosine A2A receptor bound to a miniGs heterotrimer==
<SX load='6gdg' size='340' side='right' viewer='molstar' caption='[[6gdg]], [[Resolution|resolution]] 4.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gdg]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GDG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.11&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NEC:N-ETHYL-5-CARBOXAMIDO+ADENOSINE'>NEC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gdg OCA], [https://pdbe.org/6gdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gdg RCSB], [https://www.ebi.ac.uk/pdbsum/6gdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gdg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/THIO_ECOLI THIO_ECOLI] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.[https://www.uniprot.org/uniprot/AA2AR_HUMAN AA2AR_HUMAN] Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
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== Publication Abstract from PubMed ==
The adenosine A2A receptor (A2AR) is a prototypical G protein-coupled receptor (GPCR) that couples to the heterotrimeric G protein GS. Here we determine the structure by electron cryo-microscopy (cryo-EM) of A2AR at pH 7.5 bound to the small molecule agonist NECA and coupled to an engineered heterotrimeric G protein, which contains mini-GS, the betagamma subunits and nanobody Nb35. Most regions of the complex have a resolution of ~3.8 A or better. Comparison with the 3.4 A resolution crystal structure shows that the receptor and mini-GS are virtually identical and that the density of the side chains and ligand are of comparable quality. However, the cryo-EM density map also indicates regions that are flexible in comparison to the crystal structures, which unexpectedly includes regions in the ligand binding pocket. In addition, an interaction between intracellular loop 1 of the receptor and the beta subunit of the G protein was observed.

Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein.,Garcia-Nafria J, Lee Y, Bai X, Carpenter B, Tate CG Elife. 2018 May 4;7. pii: 35946. doi: 10.7554/eLife.35946. PMID:29726815<ref>PMID:29726815</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6gdg" style="background-color:#fffaf0;"></div>

==See Also==
*[[Adenosine A2A receptor 3D structures|Adenosine A2A receptor 3D structures]]
*[[Transducin 3D structures|Transducin 3D structures]]
== References ==
<references/>
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[[Category: Escherichia coli]]
[[Category: Homo sapiens]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Garcia-Nafria J]]
[[Category: Lee Y]]