View source for 6goa


==Structural basis for OXA-48 dimerization - R189A mutant==
<StructureSection load='6goa' size='340' side='right' caption='[[6goa]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6goa]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GOA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GOA FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bla OXA-48, blaOXA-48, KPE71T_00045 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6goa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6goa OCA], [http://pdbe.org/6goa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6goa RCSB], [http://www.ebi.ac.uk/pdbsum/6goa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6goa ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many class D beta-lactamases form dimers in solution. The functional basis of the dimerization of OXA-48-like class D beta-lactamases is not known, but in order to understand the structural requirements for dimerization of OXA-48 we have characterized the dimer interface. Size-exclusion chromatography, small angle X-ray scattering and nuclear magnetic resonance were used to confirm the oligomeric state of OXA-48 in solution. X-ray crystallographic structures were used to elucidate the key interactions of dimerization. In silico residue scanning combined with site-directed mutagenesis was used to probe hot-spots of dimerization. The affinity of dimerization was quantified using microscale thermophoresis, and the overall thermostability was investigated using differential scanning calorimetry. OXA-48 was consistently found to be a dimer in solution regardless of the method used, and the biological assembly found from the SAXS envelope, is consistent with the dimer identified from the crystal structures. The buried chloride that interacts with Arg206 and Arg206' at the dimer interface was found to enhance the thermal stability by &gt;4 degrees C and crystal structures and mutations (R189A, R189A/R206A) identified several additional important ionic interactions. The affinity for OXA-48 R206A dimerization was in the picomolar range, thus reveal very high dimer affinity. In summary, OXA-48 has a very stable dimer interface, facilitated by non-covalent and predominantly charged interactions, which is stronger than the dimer interfaces previously described for other class D beta-lactamases. This article is protected by copyright. All rights reserved.

The biological assembly of OXA-48 reveals a dimer interface with high charge complementarity and very high affinity.,Lund BA, Thomassen AM, Nesheim BHB, Carlsen TJO, Isaksson J, Christopeit T, Leiros HS FEBS J. 2018 Aug 28. doi: 10.1111/febs.14643. PMID:30153368<ref>PMID:30153368</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6goa" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Beta-lactamase]]
[[Category: Leiros, H K.S]]
[[Category: Lund, B A]]
[[Category: Thomassen, A M]]
[[Category: Antibiotic]]
[[Category: Carbapenemase]]
[[Category: Dimerization]]
[[Category: Hydrolase]]
[[Category: Oxa-48]]
[[Category: Oxacillinase]]