View source for 6goy


==Structure of mEos4b in the green fluorescent state==
<StructureSection load='6goy' size='340' side='right'caption='[[6goy]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6goy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GOY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5SQ:2-[2-[(1~{S})-1-AZANYL-2-(1~{H}-IMIDAZOL-4-YL)ETHYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXIDANYLIDENE-IMIDAZOL-1-YL]ETHANOIC+ACID'>5SQ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6goy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6goy OCA], [https://pdbe.org/6goy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6goy RCSB], [https://www.ebi.ac.uk/pdbsum/6goy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6goy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort.

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.,De Zitter E, Thedie D, Monkemoller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D Nat Methods. 2019 Jul 8. pii: 10.1038/s41592-019-0462-3. doi:, 10.1038/s41592-019-0462-3. PMID:31285624<ref>PMID:31285624</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6goy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lobophyllia hemprichii]]
[[Category: Adam V]]
[[Category: Bourgeois D]]
[[Category: Byrdin M]]
[[Category: De Zitter E]]
[[Category: Dedecker P]]
[[Category: Van Meervelt L]]