View source for 6gs1


==Crystal structure of peptide transporter DtpA-nanobody in MES buffer==
<StructureSection load='6gs1' size='340' side='right' caption='[[6gs1]], [[Resolution|resolution]] 3.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gs1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GS1 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dtpA, tppB, ydgR, b1634, JW1626 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gs1 OCA], [http://pdbe.org/6gs1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gs1 RCSB], [http://www.ebi.ac.uk/pdbsum/6gs1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gs1 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/DTPA_ECOLI DTPA_ECOLI]] Proton-dependent permease that transports di- and tripeptides as well as structurally related peptidomimetics such as aminocephalosporins into the cell. Has a clear preference for dipeptides and tripeptides composed of L-amino acids, and discriminates dipeptides on the basis of the position of charges within the substrate.<ref>PMID:15175316</ref> <ref>PMID:17158458</ref> <ref>PMID:18485005</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Members of the solute carrier 15 family (SLC15) transport di- and tripeptides as well as peptidomimetic drugs across the cell membrane. Structures of bacterial homologues have provided valuable information on the binding and transport of their natural substrates, but many do not transport medically relevant drugs. In contrast, a homologue from Escherichia coli, DtpA, shows a high similarity to human PepT1 (SLC15A1) in terms of ligand selectivity and transports a similar set of drugs. Here, we present the crystal structure of DtpA in ligand-free form (at 3.30 A resolution) and in complex with the antiviral prodrug valganciclovir (at 2.65 A resolution) supported by biochemical data. We show that valganciclovir unexpectedly binds with the ganciclovir moiety mimicking the N-terminal residue of a canonical peptide substrate. Based on a homology model we argue that this binding mode also applies to the human PepT1 transporter. Our results provide new insights into the binding mode of prodrugs and will assist the rational design of drugs with improved absorption rates.

Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1.,Ural-Blimke Y, Flayhan A, Strauss J, Rantos V, Bartels K, Nielsen R, Pardon E, Steyaert J, Kosinski J, Quistgaard EM, Low C J Am Chem Soc. 2019 Jan 15. doi: 10.1021/jacs.8b11343. PMID:30644743<ref>PMID:30644743</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6gs1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Camelus glama]]
[[Category: Ecoli]]
[[Category: Flayhan, A]]
[[Category: Loew, C]]
[[Category: Quistgaard, E M]]
[[Category: Ural-Blimke, Y]]
[[Category: Complex with nanobody]]
[[Category: Membrane protein]]
[[Category: Peptide transporter]]
[[Category: Slc15 family member]]