View source for 6gsa


==Core Centromere Binding Factor 3 (CBF3) with monomeric Ndc10==
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== Structural highlights ==
<table><tr><td colspan='2'>[[6gsa]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GSA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GSA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gsa OCA], [https://pdbe.org/6gsa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gsa RCSB], [https://www.ebi.ac.uk/pdbsum/6gsa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gsa ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/CBF3B_YEAST CBF3B_YEAST] Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.
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== Publication Abstract from PubMed ==
The centromere binding factor 3 (CBF3) complex binds the third centromere DNA element in organisms with point centromeres, such as S. cerevisiae. It is an essential complex for assembly of the kinetochore in these organisms, as it facilitates genetic centromere specification and allows association of all other kinetochore components. We determined high-resolution structures of the core complex of CBF3 alone and in association with a monomeric construct of Ndc10, using cryoelectron microscopy (cryo-EM). We identify the DNA-binding site of the complex and present a model in which CBF3 induces a tight bend in centromeric DNA, thus facilitating assembly of the centromeric nucleosome.

Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.,Zhang W, Lukoynova N, Miah S, Lucas J, Vaughan CK Cell Rep. 2018 Jul 17;24(3):744-754. doi: 10.1016/j.celrep.2018.06.068. PMID:30021170<ref>PMID:30021170</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Lukoynova N]]
[[Category: Vaughan CK]]
[[Category: Zhang WJ]]