View source for 6gu0


==Crystal structure of a FimH*DsG complex from E.coli F18 with bound dimannoside Man(alpha1-3)Man in space group P213==
<StructureSection load='6gu0' size='340' side='right'caption='[[6gu0]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gu0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_f18+ Escherichia coli f18+]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GU0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ECP_4655, fimh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=488477 Escherichia coli F18+])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gu0 OCA], [http://pdbe.org/6gu0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gu0 RCSB], [http://www.ebi.ac.uk/pdbsum/6gu0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gu0 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Multivalent carbohydrate-lectin interactions at host-pathogen interfaces play a crucial role in the establishment of infections. Although competitive antagonists that prevent pathogen adhesion are promising anti-microbial drugs, the molecular mechanisms underlying these complex adhesion processes are still poorly understood. Here, we characterize the interactions between the fimbrial adhesin FimH from uropathogenic Escherichia coli strains and its natural high-mannose type N-glycan binding epitopes on uroepithelial glycoproteins. Crystal structures and a detailed kinetic characterization of ligand-binding and dissociation revealed that the binding pocket of FimH evolved such that it recognizes the terminal alpha(1-2)-, alpha(1-3)- and alpha(1-6)-linked mannosides of natural high-mannose type N-glycans with similar affinity. We demonstrate that the 2,000-fold higher affinity of the domain-separated state of FimH compared to its domain-associated state is ligand-independent and consistent with a thermodynamic cycle in which ligand-binding shifts the association equilibrium between the FimH lectin and the FimH pilin domain. Moreover, we show that a single N-glycan can bind up to three molecules of FimH, albeit with negative cooperativity, so that a molar excess of accessible N-glycans over FimH on the cell surface favors monovalent FimH binding. Our data provide pivotal insights into the adhesion properties of uropathogenic Escherichia coli strains to their target receptors and a solid basis for the development of effective FimH antagonists.

Binding of the bacterial adhesin FimH to its natural, multivalent high-mannose type glycan targets.,Sauer MM, Jakob RP, Luber T, Canonica F, Navarra G, Ernst B, Unverzagt C, Maier T, Glockshuber R J Am Chem Soc. 2018 Dec 13. doi: 10.1021/jacs.8b10736. PMID:30543411<ref>PMID:30543411</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6gu0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli f18+]]
[[Category: Large Structures]]
[[Category: Canonica, F]]
[[Category: Ernst, B]]
[[Category: Glockshuber, R]]
[[Category: Jakob, R P]]
[[Category: Luber, T]]
[[Category: Maier, T]]
[[Category: Navarra, G]]
[[Category: Sauer, M M]]
[[Category: Unverzagt, C]]
[[Category: Catch-bond]]
[[Category: Cell adhesion]]
[[Category: Infection]]
[[Category: Lectin]]
[[Category: Mannose]]
[[Category: Type i pilus]]
[[Category: Upec]]