View source for 6gxj


==X-ray structure of DiRu-1-encapsulated Apoferritin==
<StructureSection load='6gxj' size='340' side='right'caption='[[6gxj]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gxj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GXJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5erk|5erk]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gxj OCA], [https://pdbe.org/6gxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gxj RCSB], [https://www.ebi.ac.uk/pdbsum/6gxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gxj ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/FRIL_HORSE FRIL_HORSE]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). 
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== Publication Abstract from PubMed ==
The effects of encapsulating the cytotoxic dinuclear trithiolato-bridged arene ruthenium complex [(eta(6) -p-MeC6 H4 iPr)2 Ru2 (mu2 -S-p-C6 H4 tBu)3 ]Cl (DiRu-1) within the apoferritin (AFt) nanocage were investigated. The DiRu-1-AFt nanocarrier was characterized by UV/Vis spectroscopy, ICP-MS, CD and X-ray crystallography. In contrast to previously reported Au- and Pt-based drug-loaded AFt carriers, we found no evidence of direct interactions between DiRu-1 and AFt. DiRu-1-AFt is cytotoxic toward immortalized murine BALB/c-3T3 fibroblasts transformed with SV40 virus (SVT2) and human epidermoid carcinoma A431 malignant cells, and exhibits moderate selectivity for these cancer cells over normal BALB/c-3T3 cells. DiRu-1-AFt triggers the production of reactive oxygen species, depolarization of mitochondrial membrane potential, and induces cell death via p53-mediated apoptosis. Comparison between our data and previous results suggests that the presence of specific interactions between a metal-based drug and AFt within the protein cage is not essential for drug encapsulation.

Encapsulation of the Dinuclear Trithiolato-Bridged Arene Ruthenium Complex Diruthenium-1 in an Apoferritin Nanocage: Structure and Cytotoxicity.,Petruk G, Monti DM, Ferraro G, Pica A, D'Elia L, Pane F, Amoresano A, Furrer J, Kowalski K, Merlino A ChemMedChem. 2019 Mar 5;14(5):594-602. doi: 10.1002/cmdc.201800805. Epub 2019 Feb, 11. PMID:30674089<ref>PMID:30674089</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6gxj" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ferritin 3D structures|Ferritin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Equus caballus]]
[[Category: Large Structures]]
[[Category: Ferraro, G]]
[[Category: Merlino, A]]
[[Category: Pica, A]]
[[Category: Ferritin nanocage]]
[[Category: Metal transport]]
[[Category: Metal-based compound encapsulation]]
[[Category: Ruthenium complex]]