View source for 6gys


==Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore==
<SX load='6gys' size='340' side='right' viewer='molstar' caption='[[6gys]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gys]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GYS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gys OCA], [https://pdbe.org/6gys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gys RCSB], [https://www.ebi.ac.uk/pdbsum/6gys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gys ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/CBF3C_YEAST CBF3C_YEAST] Acts as central component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly.
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== Publication Abstract from PubMed ==
Kinetochores are multicomponent complexes responsible for coordinating the attachment of centromeric DNA to mitotic-spindle microtubules. The point centromeres of budding yeast are organized into three centromeric determining elements (CDEs), and are associated with the centromere-specific nucleosome Cse4. Deposition of Cse4 at CEN loci is dependent on the CBF3 complex that engages CDEIII to direct Cse4 nucleosomes to CDEII. To understand how CBF3 recognizes CDEIII and positions Cse4, we determined a cryo-EM structure of a CBF3-CEN complex. CBF3 interacts with CEN DNA as a head-to-head dimer that includes the whole of CDEIII and immediate 3' regions. Specific CEN-binding of CBF3 is mediated by a Cep3 subunit of one of the CBF3 protomers that forms major groove interactions with the conserved and essential CCG and TGT motifs of CDEIII. We propose a model for a CBF3-Cse4-CEN complex with implications for understanding CBF3-directed deposition of the Cse4 nucleosome at CEN loci.

Architecture of the CBF3-centromere complex of the budding yeast kinetochore.,Yan K, Zhang Z, Yang J, McLaughlin SH, Barford D Nat Struct Mol Biol. 2018 Nov 26. pii: 10.1038/s41594-018-0154-1. doi:, 10.1038/s41594-018-0154-1. PMID:30478265<ref>PMID:30478265</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Centromere protein 3D structure|Centromere protein 3D structure]]
== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Barford D]]
[[Category: McLaughlin SH]]
[[Category: Yan K]]
[[Category: Yang J]]
[[Category: Zhang Z]]