View source for 6hc3


==TFAM bound to Site-X==
<StructureSection load='6hc3' size='340' side='right'caption='[[6hc3]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hc3]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HC3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HC3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TFAM, TCF6, TCF6L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hc3 OCA], [http://pdbe.org/6hc3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hc3 RCSB], [http://www.ebi.ac.uk/pdbsum/6hc3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hc3 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/TFAM_HUMAN TFAM_HUMAN]] Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.<ref>PMID:1737790</ref> <ref>PMID:20410300</ref> <ref>PMID:19304746</ref> <ref>PMID:22037172</ref> <ref>PMID:22037171</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human mitochondrial DNA (h-mtDNA) codes for 13 subunits of the oxidative phosphorylation pathway, the essential route that produces ATP. H-mtDNA transcription and replication depends on the transcription factor TFAM, which also maintains and compacts this genome. It is well-established that TFAM activates the mtDNA promoters LSP and HSP1 at the mtDNA control region where DNA regulatory elements cluster. Previous studies identified still uncharacterized, additional binding sites at the control region downstream from and slightly similar to LSP, namely sequences X and Y (Site-X and Site-Y) (Fisher et al., Cell 50, pp 247-258, 1987). Here, we explore TFAM binding at these two sites and compare them to LSP by multiple experimental and in silico methods. Our results show that TFAM binding is strongly modulated by the sequence-dependent properties of Site-X, Site-Y and LSP. The high binding versatility of Site-Y or the considerable stiffness of Site-X tune TFAM interactions. In addition, we show that increase in TFAM/DNA complex concentration induces multimerization, which at a very high concentration triggers disruption of preformed complexes. Therefore, our results suggest that mtDNA sequences induce non-uniform TFAM binding and, consequently, direct an uneven distribution of TFAM aggregation sites during the essential process of mtDNA compaction.

DNA specificities modulate the binding of human transcription factor A to mitochondrial DNA control region.,Cuppari A, Fernandez-Millan P, Battistini F, Tarres-Sole A, Lyonnais S, Iruela G, Ruiz-Lopez E, Enciso Y, Rubio-Cosials A, Prohens R, Pons M, Alfonso C, Toth K, Rivas G, Orozco M, Sola M Nucleic Acids Res. 2019 May 22. pii: 5494731. doi: 10.1093/nar/gkz406. PMID:31114891<ref>PMID:31114891</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6hc3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Cuppari, A]]
[[Category: Fernandez-Millan, P]]
[[Category: Lyonnais, S]]
[[Category: Rubio-Cosials, A]]
[[Category: Sola, M]]
[[Category: Tarres-Sole, A]]
[[Category: Dna binding protein]]
[[Category: Dna compaction]]
[[Category: Mitochondria]]
[[Category: Mitochondrial dna]]
[[Category: Transcription factor]]