View source for 6hfg


==Structure of the REC114 PH domain==
<StructureSection load='6hfg' size='340' side='right'caption='[[6hfg]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hfg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HFG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HFG FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hfg OCA], [http://pdbe.org/6hfg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hfg RCSB], [http://www.ebi.ac.uk/pdbsum/6hfg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hfg ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/RE114_MOUSE RE114_MOUSE]] Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination (PubMed:20551173, PubMed:27723721). Probably acts by forming a complex with IHO1/CCDC36 and MEI4, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis (PubMed:27723721).<ref>PMID:20551173</ref> <ref>PMID:27723721</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Programmed formation of DNA double-strand breaks (DSBs) initiates the meiotic homologous recombination pathway. This pathway is essential for proper chromosome segregation at the first meiotic division and fertility. Meiotic DSBs are catalyzed by Spo11. Several other proteins are essential for meiotic DSB formation, including three evolutionarily conserved proteins first identified in Saccharomyces cerevisiae (Mer2, Mei4, and Rec114). These three S. cerevisiae proteins and their mouse orthologs (IHO1, MEI4, and REC114) co-localize on the axes of meiotic chromosomes, and mouse IHO1 and MEI4 are essential for meiotic DSB formation. Here, we show that mouse Rec114 is required for meiotic DSB formation. Moreover, MEI4 forms a complex with REC114 and IHO1 in mouse spermatocytes, consistent with cytological observations. We then demonstrated in vitro the formation of a stable complex between REC114 C-terminal domain and MEI4 N-terminal domain. We further determine the structure of the REC114 N-terminal domain that revealed similarity with Pleckstrin homology domains. These analyses provide direct insights into the architecture of these essential components of the meiotic DSB machinery.

Mouse REC114 is essential for meiotic DNA double-strand break formation and forms a complex with MEI4.,Kumar R, Oliver C, Brun C, Juarez-Martinez AB, Tarabay Y, Kadlec J, de Massy B Life Sci Alliance. 2018 Dec 10;1(6):e201800259. doi: 10.26508/lsa.201800259., eCollection 2018 Dec. PMID:30569039<ref>PMID:30569039</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6hfg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Juarez-Martinez, A B]]
[[Category: Kadlec, J]]
[[Category: Massy, B de]]
[[Category: Pleckstrin homology domain meiotic recombination]]
[[Category: Recombination]]