View source for 6hmg


==Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-Glc-1,3-(1,2-anhydro-carba-glucosamine)==
<StructureSection load='6hmg' size='340' side='right'caption='[[6hmg]], [[Resolution|resolution]] 1.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hmg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_xylanisolvens_XB1A Bacteroides xylanisolvens XB1A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HMG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HMG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.27&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GDQ:(1~{S},2~{R},3~{R},4~{R},6~{S})-4-(hydroxymethyl)-7-azabicyclo[4.1.0]heptane-2,3-diol'>GDQ</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hmg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hmg OCA], [https://pdbe.org/6hmg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hmg RCSB], [https://www.ebi.ac.uk/pdbsum/6hmg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hmg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D6D1V7_9BACE D6D1V7_9BACE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Understanding the enzyme reaction mechanism can lead to the design of enzyme inhibitors. A Claisen rearrangement was used to allow conversion of an alpha-1,4-disaccharide into an alpha-1,3-linked glycosyl carbasugar to target the endo-alpha-mannosidase from the GH99 glycosidase family, which, unusually, is believed to act through a 1,2-anhydrosugar "epoxide" intermediate. Using NMR and X-ray crystallography, it is shown that glucosyl carbasugar alpha-aziridines can act as reasonably potent endo-alpha-mannosidase inhibitors, likely by virtue of their shape mimicry and the interactions of the aziridine nitrogen with the conserved catalytic acid/base of the enzyme active site.

From 1,4-Disaccharide to 1,3-Glycosyl Carbasugar: Synthesis of a Bespoke Inhibitor of Family GH99 Endo-alpha-mannosidase.,Lu D, Zhu S, Sobala LF, Bernardo-Seisdedos G, Millet O, Zhang Y, Jimenez-Barbero J, Davies GJ, Sollogoub M Org Lett. 2018 Nov 14. doi: 10.1021/acs.orglett.8b03260. PMID:30427198<ref>PMID:30427198</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6hmg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacteroides xylanisolvens XB1A]]
[[Category: Large Structures]]
[[Category: Bernardo-Seisdedos G]]
[[Category: Davies GJ]]
[[Category: Jimenez-Barbero J]]
[[Category: Lu D]]
[[Category: Millet O]]
[[Category: Sobala LF]]
[[Category: Sollogoub M]]
[[Category: Zhang Y]]
[[Category: Zhu S]]