View source for 6hnu


==Crystal structure of the aminotransferase Aro8 from C. Albicans with ligands==
<StructureSection load='6hnu' size='340' side='right'caption='[[6hnu]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hnu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_WO-1 Candida albicans WO-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HNU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hnu OCA], [https://pdbe.org/6hnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hnu RCSB], [https://www.ebi.ac.uk/pdbsum/6hnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hnu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C4YJ02_CANAW C4YJ02_CANAW] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aminotransferases catalyze reversibly the transamination reaction by a ping-pong bi-bi mechanism with pyridoxal 5'-phosphate (PLP) as a cofactor. Various aminotransferases acting on a range of substrates have been reported. Aromatic transaminases are able to catalyze the transamination reaction with both aromatic and acidic substrates. Two aminotransferases from C. albicans, Aro8p and Aro9p, have been identified recently, exhibiting different catalytic properties. To elucidate the multiple substrate recognition of the two enzymes we determined the crystal structures of an unliganded CaAro8p, a complex of CaAro8p with the PLP cofactor bound to a substrate, forming an external aldimine, CaAro9p with PLP in the form of internal aldimine, and CaAro9p with a mixture of ligands that have been interpreted as results of the enzymatic reaction. The crystal structures of both enzymes contains in the asymmetric unit a biologically relevant dimer of 55kDa for CaAro8 and 59kDa for CaAro9p protein subunits. The ability of the enzymes to process multiple substrates could be related to a feature of their architecture in which the active site resides on one subunit while the substrate-binding site is formed by a long loop extending from the other subunit of the dimeric molecule. The separation of the two functions to different chemical entities could facilitate the evolution of the substrate-binding part and allow it to be flexible without destabilizing the conservative catalytic mechanism.

Crystal structures of aminotransferases Aro8 and Aro9 from Candida albicans and structural insights into their properties.,Kiliszek A, Rypniewski W, Rzad K, Milewski S, Gabriel I J Struct Biol. 2019 Feb 8. pii: S1047-8477(19)30013-9. doi:, 10.1016/j.jsb.2019.02.001. PMID:30742897<ref>PMID:30742897</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6hnu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Candida albicans WO-1]]
[[Category: Large Structures]]
[[Category: Gabriel I]]
[[Category: Kiliszek A]]
[[Category: Milewski S]]
[[Category: Rypniewski W]]
[[Category: Rzad K]]