View source for 6htj


==Crystal structure of the translation recovery factor Trf from Sulfolobus solfataricus==
<StructureSection load='6htj' size='340' side='right'caption='[[6htj]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6htj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HTJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSOP1_2634, SULA_0310, SULB_0312, SULC_0310 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6htj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6htj OCA], [http://pdbe.org/6htj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6htj RCSB], [http://www.ebi.ac.uk/pdbsum/6htj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6htj ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
During translation initiation, the heterotrimeric archaeal translation initiation factor 2 (aIF2) recruits the initiator tRNAi to the small ribosomal subunit. In the stationary growth phase and/or during nutrient stress, Sulfolobus solfataricus aIF2 has a second function: it protects leaderless mRNAs against degradation by binding to their 5'-ends. The S. solfataricus protein Sso2509 is a translation recovery factor (Trf) that interacts with aIF2 and is responsible for the release of aIF2 from bound mRNAs, thereby enabling translation re-initiation. It is a member of the DUF35 protein family and is conserved in Sulfolobales as well as in other archaea. Here, we present the X-ray structure of S. solfataricus Trf solved to a resolution of 1.65 A. Trf is composed of an N-terminal rubredoxin-like domain containing a bound zinc ion and a C-terminal OB-fold domain. The Trf structure reveals putative mRNA binding sites in both domains. Surprisingly, the Trf protein is structurally but not sequentially very similar to proteins linked to acyl-CoA utilization - e. g. the Sso2064 protein from Sulfolobus solfataricus, as well as to scaffold proteins found in the acetoacetyl-CoA thiolase/HMG-CoA synthase complex of the archaeon Methanothermococcus thermolithotrophicus, and in a steroid side chain cleaving aldolase complex from the bacterium Thermomonospora curvata. This suggests that members of the DUF35 protein family are able to act as scaffolding and binding proteins in a wide variety of biological processes.

Crystal structure of the translation recovery factor Trf from Sulfolobus solfataricus.,Kaiser M, Wurm JP, Martens B, Blasi U, Pogoryelov D, Wohnert J FEBS Open Bio. 2019 Dec 5. doi: 10.1002/2211-5463.12772. PMID:31804766<ref>PMID:31804766</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6htj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Atcc 35091]]
[[Category: Large Structures]]
[[Category: Kaiser, M]]
[[Category: Pogoryelov, D]]
[[Category: Woehnert, J]]
[[Category: Ribosome]]
[[Category: Sulfolobus solfataricus]]
[[Category: Translation]]
[[Category: Translation initiation]]
[[Category: Translation recovery factor trf]]