View source for 6hvl


==CdaA complex with c-di-AMP and AMP==
<StructureSection load='6hvl' size='340' side='right'caption='[[6hvl]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hvl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HVL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2BA:(2R,3R,3AS,5R,7AR,9R,10R,10AS,12R,14AR)-2,9-BIS(6-AMINO-9H-PURIN-9-YL)OCTAHYDRO-2H,7H-DIFURO[3,2-D 3,2-J][1,3,7,9,2,8]TETRAOXADIPHOSPHACYCLODODECINE-3,5,10,12-TETROL+5,12-DIOXIDE'>2BA</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hvl OCA], [https://pdbe.org/6hvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hvl RCSB], [https://www.ebi.ac.uk/pdbsum/6hvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hvl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DACA_LISMO DACA_LISMO] Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP), a signaling compound secreted into the host's cytosol where it triggers the cytosolic surveillance pathway (CSP), a host pathway of innate immunity characterized by expression of beta interferon (IFN-beta) and coregulated genes.<ref>PMID:20508090</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cyclic di-AMP (c-di-AMP) is the only second messenger known to be essential for bacterial growth. It has been mainly found in gram-positive bacteria, including pathogenic bacteria like Listeria monocytogenes. CdaA is the sole di-adenylate cyclase in L. monocytogenes rendering this enzyme an attractive target for the development of novel antibiotic compounds. Here we report crystal structures of CdaA from L. monocytogenes in the apo-state, in the post-catalytic state with bound c-di-AMP and catalytic Co2+ ions, as well as in a complex with AMP. These structures reveal the flexibility of a tyrosine side-chain involved in locking the adenine ring after ATP binding. The essential role of this tyrosine was confirmed by mutation to Ala leading to a drastic loss of enzymatic activity.

Crystal structures of the c-di-AMP synthesizing enzyme CdaA.,Heidemann JL, Neumann P, Dickmanns A, Ficner R J Biol Chem. 2019 May 22. pii: RA119.009246. doi: 10.1074/jbc.RA119.009246. PMID:31118276<ref>PMID:31118276</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6hvl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Listeria monocytogenes EGD-e]]
[[Category: Ficner R]]
[[Category: Heidemann JL]]
[[Category: Neumann P]]