View source for 6hxv


==Crystal structure of the head and coiled-coil domains of zebrafish CCDC61 (F129E/D130A)==
<StructureSection load='6hxv' size='340' side='right'caption='[[6hxv]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hxv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HXV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hxv OCA], [https://pdbe.org/6hxv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hxv RCSB], [https://www.ebi.ac.uk/pdbsum/6hxv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hxv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CCD61_DANRE CCD61_DANRE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Centrioles are cylindrical assemblies whose peripheral microtubule array displays a 9-fold rotational symmetry that is established by the scaffolding protein SAS6. Centriole symmetry can be broken by centriole-associated structures, such as the striated fibers in Chlamydomonas that are important for ciliary function. The conserved protein CCDC61/VFL3 is involved in this process, but its exact role is unclear. Here, we show that CCDC61 is a paralog of SAS6. Crystal structures of CCDC61 demonstrate that it contains two homodimerization interfaces that are similar to those found in SAS6, but result in the formation of linear filaments rather than rings. Furthermore, we show that CCDC61 binds microtubules and that residues involved in CCDC61 microtubule binding are important for ciliary function in Chlamydomonas. Together, our findings suggest that CCDC61 and SAS6 functionally diverged from a common ancestor while retaining the ability to scaffold the assembly of basal body-associated structures or centrioles, respectively.

CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions.,Ochi T, Quarantotti V, Lin H, Jullien J, Rosa E Silva I, Boselli F, Barnabas DD, Johnson CM, McLaughlin SH, Freund SMV, Blackford AN, Kimata Y, Goldstein RE, Jackson SP, Blundell TL, Dutcher SK, Gergely F, van Breugel M Structure. 2020 May 5. pii: S0969-2126(20)30131-3. doi:, 10.1016/j.str.2020.04.010. PMID:32375023<ref>PMID:32375023</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6hxv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Danio rerio]]
[[Category: Large Structures]]
[[Category: Barnabas D]]
[[Category: Ochi T]]
[[Category: Van Breugel M]]