View source for 6hyu


==Crystal structure of DHX8 helicase bound to single stranded poly-adenine RNA==
<StructureSection load='6hyu' size='340' side='right'caption='[[6hyu]], [[Resolution|resolution]] 3.22&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hyu]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HYU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HYU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hyu OCA], [http://pdbe.org/6hyu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hyu RCSB], [http://www.ebi.ac.uk/pdbsum/6hyu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hyu ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/DHX8_HUMAN DHX8_HUMAN]] Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.<ref>PMID:8608946</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DHX8 is a crucial DEAH-box RNA helicase involved in splicing and required for the release of mature mRNA from the spliceosome. Here we report the biochemical characterization of full-length human DHX8 and the catalytically active helicase core DHX8Delta547, alongside crystal structures of DHX8Delta547 bound to ADP and a structure of DHX8Delta547 bound to poly(A)6 single-strand RNA. Our results reveal that DHX8 has an in vitro binding preference for adenine-rich RNA and that RNA binding triggers release of ADP through significant conformational flexibility in the conserved DEAH-, P-loop and hook-turn motifs. We demonstrate the importance of R620 and both the hook-turn and hook-loop regions for DHX8 helicase activity, and propose that the hook-turn acts as a gatekeeper to regulate directional movement of the 3' end of RNA through the RNA binding channel. This study provides an in-depth understanding of the activity of DHX8 and contributes insights into the RNA unwinding mechanisms of the DEAH-box helicase family.

Structural and functional characterisation of human RNA helicase DHX8 provides insights into the mechanism of RNA-stimulated ADP release.,Felisberto-Rodrigues C, Thomas JC, McAndrew C, Le Bihan YV, Burke R, Workman P, van Montfort RLM Biochem J. 2019 Aug 13. pii: BCJ20190383. doi: 10.1042/BCJ20190383. PMID:31409651<ref>PMID:31409651</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6hyu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: RNA helicase]]
[[Category: Bihan, Y V.Le]]
[[Category: Burke, R]]
[[Category: Felisberto-Rodrigues, C]]
[[Category: McAndrew, P C]]
[[Category: Montfort, R L.M van]]
[[Category: Thomas, J C]]
[[Category: Workman, P]]
[[Category: Helicase]]
[[Category: Rna]]
[[Category: Rna binding protein]]
[[Category: Splicing]]