View source for 6i43


==SFX Structure of Damage Free Ferric State of Dye Type Peroxidase Aa from Streptomyces lividans==
<StructureSection load='6i43' size='340' side='right'caption='[[6i43]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6i43]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I43 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i43 OCA], [https://pdbe.org/6i43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i43 RCSB], [https://www.ebi.ac.uk/pdbsum/6i43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i43 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9RKQ2_STRCO Q9RKQ2_STRCO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
An approach is demonstrated to obtain, in a sample- and time-efficient manner, multiple dose-resolved crystal structures from room-temperature protein microcrystals using identical fixed-target supports at both synchrotrons and X-ray free-electron lasers (XFELs). This approach allows direct comparison of dose-resolved serial synchrotron and damage-free XFEL serial femtosecond crystallography structures of radiation-sensitive proteins. Specifically, serial synchrotron structures of a heme peroxidase enzyme reveal that X-ray induced changes occur at far lower doses than those at which diffraction quality is compromised (the Garman limit), consistent with previous studies on the reduction of heme proteins by low X-ray doses. In these structures, a functionally relevant bond length is shown to vary rapidly as a function of absorbed dose, with all room-temperature synchrotron structures exhibiting linear deformation of the active site compared with the XFEL structure. It is demonstrated that extrapolation of dose-dependent synchrotron structures to zero dose can closely approximate the damage-free XFEL structure. This approach is widely applicable to any protein where the crystal structure is altered by the synchrotron X-ray beam and provides a solution to the urgent requirement to determine intact structures of such proteins in a high-throughput and accessible manner.

Dose-resolved serial synchrotron and XFEL structures of radiation-sensitive metalloproteins.,Ebrahim A, Moreno-Chicano T, Appleby MV, Chaplin AK, Beale JH, Sherrell DA, Duyvesteyn HME, Owada S, Tono K, Sugimoto H, Strange RW, Worrall JAR, Axford D, Owen RL, Hough MA IUCrJ. 2019 May 3;6(Pt 4):543-551. doi: 10.1107/S2052252519003956. eCollection, 2019 Jul 1. PMID:31316799<ref>PMID:31316799</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6i43" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Chaplin AK]]
[[Category: Duyvesteyn HME]]
[[Category: Ebrahim A]]
[[Category: Moreno-Chicano T]]
[[Category: Owada S]]
[[Category: Sherrell DA]]
[[Category: Strange RW]]
[[Category: Sugimoto H]]
[[Category: Tono K]]
[[Category: Worrall JAR]]