View source for 6iae


==T. brucei IFT22 GDP-bound crystal structure==
<StructureSection load='6iae' size='340' side='right'caption='[[6iae]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6iae]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tryb2 Tryb2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IAE FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ia7|6ia7]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IFT22, Tb11.01.8590 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 TRYB2])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iae OCA], [http://pdbe.org/6iae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iae RCSB], [http://www.ebi.ac.uk/pdbsum/6iae PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iae ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/IFT22_TRYB2 IFT22_TRYB2]] Required for flagellum formation.<ref>PMID:19240117</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in Trypanosoma brucei although IFT22 GTP-loading is not strictly required.

Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly.,Wachter S, Jung J, Shafiq S, Basquin J, Fort C, Bastin P, Lorentzen E EMBO J. 2019 Apr 2. pii: embj.2018101251. doi: 10.15252/embj.2018101251. PMID:30940671<ref>PMID:30940671</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6iae" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tryb2]]
[[Category: Basquin, J]]
[[Category: Lorentzen, E]]
[[Category: Wachter, S]]
[[Category: Cilium formation]]
[[Category: Cytosolic protein]]
[[Category: Gtpase]]
[[Category: Intraflagellar transport protein 22]]
[[Category: Rab-like]]
[[Category: Rabl5]]