View source for 6ifm


==Crystal structure of DNA bound VapBC from Salmonella typhimurium==
<StructureSection load='6ifm' size='340' side='right'caption='[[6ifm]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ifm]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IFM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.804&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ifm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifm OCA], [https://pdbe.org/6ifm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ifm RCSB], [https://www.ebi.ac.uk/pdbsum/6ifm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VAPC_SALTY VAPC_SALTY] Toxic component of a type II toxin-antitoxin (TA) system. A site-specific tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Does not cleave tRNA(Met), tRNA(Arg2), tRNA(His), tRNA(Leu), tRNA(Phe) tRNA(Thr1), tRNA(Tyr) or tRNA(Val). Overexpression in E.coli inhibits translation, leads to loss of cell growth and degradation of tRNA(fMet), these effects are neutralized by expression of cognate antitoxin VapB. Expression also activates translation initiation at correctly positioned elongator codons (AAA and to a lesser extent AAG).  Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity.

==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
[[Category: Synthetic construct]]
[[Category: Lee BJ]]
[[Category: Park DW]]