View source for 6ihd


==Crystal structure of Malate dehydrogenase from Metallosphaera sedula==
<StructureSection load='6ihd' size='340' side='right'caption='[[6ihd]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ihd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Metallosphaera_sedula Metallosphaera sedula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IHD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ETX:2-ETHOXYETHANOL'>ETX</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ihd OCA], [https://pdbe.org/6ihd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ihd RCSB], [https://www.ebi.ac.uk/pdbsum/6ihd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ihd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A088E2H7_9CREN A0A088E2H7_9CREN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Metallosphaera sedula is a thermoacidophilic autotrophic archaeon and known to utilize the 3-hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as a carbon fixation pathway. The 3-HP/4-HB cycle in M. sedula is associated with central metabolism, and malate dehydrogenase (MDH) is an enzyme involved in the central metabolism that converts malate to oxaloacetate. To elucidate the enzymatic properties of MDH from M. sedula (MsMDH), we determined the crystal structure of MsMDH as a complex with NAD(+) and a ternary complex with malate and NAD(+). Based on its complex structures and biochemical experiments, we observed that MsMDH can utilize both NAD(+) and NADP(+) as a cofactor. In addition, we revealed that MsMDH shows a conformational change at the active site upon substrate binding. Based on the comparison with other MDHs, we revealed that MsMDH was distinguished from general MDHs due to a Lys80 residue, and this difference is likely to influence the unique cofactor specificity of MsMDH.

Crystal structure and biochemical characterization of malate dehydrogenase from Metallosphaera sedula.,Lee D, Hong J, Kim KJ Biochem Biophys Res Commun. 2019 Feb 12;509(3):833-838. doi:, 10.1016/j.bbrc.2019.01.018. Epub 2019 Jan 10. PMID:30638660<ref>PMID:30638660</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ihd" style="background-color:#fffaf0;"></div>

==See Also==
*[[Malate Dehydrogenase 3D structures|Malate Dehydrogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Metallosphaera sedula]]
[[Category: Kim KJ]]
[[Category: Lee D]]