View source for 6ii2


==Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O==
<StructureSection load='6ii2' size='340' side='right'caption='[[6ii2]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ii2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6II2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6II2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ii2 OCA], [https://pdbe.org/6ii2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ii2 RCSB], [https://www.ebi.ac.uk/pdbsum/6ii2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ii2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F1CLG9_VIBVL F1CLG9_VIBVL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Upon invading target cells, multifunctional autoprocessing repeats-in-toxin (MARTX) toxins secreted by bacterial pathogens release their disease-related modularly structured effector domains. However, it is unclear how a diverse repertoire of effector domains within these toxins are processed and activated. Here, we report that Makes caterpillars floppy-like effector (MCF)-containing MARTX toxins require ubiquitous ADP-ribosylation factor (ARF) proteins for processing and activation of intermediate effector modules, which localize in different subcellular compartments following limited processing of holo effector modules by the internal cysteine protease. Effector domains structured tandemly with MCF in intermediate modules become disengaged and fully activated by MCF, which aggressively interacts with ARF proteins present at the same location as intermediate modules and is converted allosterically into a catalytically competent protease. MCF-mediated effector processing leads ultimately to severe virulence in mice via an MCF-mediated ARF switching mechanism across subcellular compartments. This work provides insight into how bacteria take advantage of host systems to induce systemic pathogenicity.

Makes caterpillars floppy-like effector-containing MARTX toxins require host ADP-ribosylation factor (ARF) proteins for systemic pathogenicity.,Lee Y, Kim BS, Choi S, Lee EY, Park S, Hwang J, Kwon Y, Hyun J, Lee C, Kim JF, Eom SH, Kim MH Proc Natl Acad Sci U S A. 2019 Sep 3;116(36):18031-18040. doi:, 10.1073/pnas.1905095116. Epub 2019 Aug 19. PMID:31427506<ref>PMID:31427506</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ii2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio vulnificus]]
[[Category: Choi S]]
[[Category: Eom SH]]
[[Category: Hwang J]]
[[Category: Hyung J]]
[[Category: Kim BS]]
[[Category: Kim MH]]
[[Category: Kwon Y]]
[[Category: Lee C]]
[[Category: Lee EY]]
[[Category: Lee Y]]
[[Category: Park S]]