View source for 6imw


==The complex structure of endo-beta-1,2-glucanase mutant (E262Q) from Talaromyces funiculosus with beta-1,2-glucan==
<StructureSection load='6imw' size='340' side='right'caption='[[6imw]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6imw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Talaromyces_funiculosus Talaromyces funiculosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IMW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6imw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6imw OCA], [https://pdbe.org/6imw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6imw RCSB], [https://www.ebi.ac.uk/pdbsum/6imw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6imw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A4V8H013_TALFU A0A4V8H013_TALFU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
endo-beta-1,2-Glucanase (SGL) is an enzyme that hydrolyzes beta-1,2-glucans, which play important physiological roles in some bacteria as a cyclic form. To date, no eukaryotic SGL has been identified. We purified an SGL from Talaromyces funiculosus (TfSGL), a soil fungus, to homogeneity and then cloned the complementary DNA encoding the enzyme. TfSGL shows no significant sequence similarity to any known glycoside hydrolase (GH) families, but significant homology to certain eukaryotic proteins with unknown functions. The recombinant TfSGL (TfSGLr) specifically hydrolyzed linear and cyclic beta-1,2-glucans to sophorose (Glc-beta-1,2-Glc) as a main product. TfSGLr hydrolyzed reducing-end-modified beta-1,2-glucooligosaccharides to release a sophoroside with the modified moiety. These results indicate that TfSGL is an endo-type enzyme that preferably releases sophorose from the reducing end of substrates. Stereochemical analysis demonstrated that TfSGL is an inverting enzyme. The overall structure of TfSGLr includes an (alpha/alpha)6 toroid fold. The substrate binding mode was revealed by the structure of a Michaelis complex of an inactive TfSGLr-mutant with a beta-1,2-glucoheptasaccharide. Mutational analysis and action pattern analysis of beta-1,2-glucooligosaccharide derivatives revealed an unprecedented catalytic mechanism for substrate hydrolysis. Glu-262 (general acid) indirectly protonates the anomeric oxygen at subsite -1 via the 3-hydroxy group of the Glc moiety at subsite +2, and Asp-446 (general base) activates the nucleophilic water via another water. TfSGLr is apparently different from a GH144 SGL in the reaction and substrate recognition mechanism based on structural comparison. Overall, we propose that TfSGL and closely related GH enzymes can be classified into a new family, GHxyz.

Identification, characterization and structural analyses of a fungal endo-beta-1,2-glucanase reveal a new glycoside hydrolase family.,Tanaka N, Nakajima M, Narukawa-Nara M, Matsunaga H, Kamisuki S, Aramasa H, Takahashi Y, Sugimoto N, Abe K, Terada T, Miyanaga A, Yamashita T, Sugawara F, Kamakura T, Komba S, Nakai H, Taguchi H J Biol Chem. 2019 Mar 29. pii: RA118.007087. doi: 10.1074/jbc.RA118.007087. PMID:30926603<ref>PMID:30926603</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6imw" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Talaromyces funiculosus]]
[[Category: Abe K]]
[[Category: Aramasa H]]
[[Category: Kamakura T]]
[[Category: Kamisuki S]]
[[Category: Komba S]]
[[Category: Matsunaga H]]
[[Category: Miyanaga A]]
[[Category: Nakai H]]
[[Category: Nakajima M]]
[[Category: Narukawa-Nara M]]
[[Category: Sugawara F]]
[[Category: Sugimoto N]]
[[Category: Taguchi H]]
[[Category: Takahashi Y]]
[[Category: Tanaka N]]
[[Category: Yamashita T]]