View source for 6io4


==Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A==
<StructureSection load='6io4' size='340' side='right'caption='[[6io4]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6io4]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IO4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6io4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6io4 OCA], [https://pdbe.org/6io4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6io4 RCSB], [https://www.ebi.ac.uk/pdbsum/6io4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6io4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G3P1_ECOLI G3P1_ECOLI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Silver has long been used as an antibacterial agent, yet its molecular targets remain largely unknown. Using a custom-designed coupling of gel electrophoresis with inductively coupled plasma mass spectrometry (GE-ICP-MS), we identified six silver-binding proteins in E. coli. The majority of the identified proteins are associated with the central carbon metabolism of E. coli. Among them, we unveil that GAPDH, an essential enzyme in glycolysis, serves as a vital target of Ag(+) in E. coli for the first time. We demonstrate that silver inhibits the enzymatic function of GAPDH through targeting Cys149 in its catalytic site. The X-ray structure reveals that Ag(+) coordinates to Cys149 and His176 with a quasi-linear geometry (S-Ag-N angle of 157 degrees ). And unexpectedly, two Ag(+) ions coordinate to Cys288 in the non-catalytic site with weak argentophilic interaction (Ag...Ag distance of 2.9 A). This is the first report on antimicrobial Ag(+) targeting a key enzyme in the glycolytic pathway of E. coli. The findings expand our knowledge on the mode of action and bio-coordination chemistry of silver, particularly silver-targeting residues in proteins at the atomic level.

Antimicrobial silver targets glyceraldehyde-3-phosphate dehydrogenase in glycolysis of E. coli.,Wang H, Wang M, Yang X, Xu X, Hao Q, Yan A, Hu M, Lobinski R, Li H, Sun H Chem Sci. 2019 Jun 24;10(30):7193-7199. doi: 10.1039/c9sc02032b. eCollection 2019, Aug 14. PMID:31588287<ref>PMID:31588287</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6io4" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glyceraldehyde-3-phosphate dehydrogenase 3D structures|Glyceraldehyde-3-phosphate dehydrogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Sun H]]
[[Category: Wang H]]
[[Category: Wang M]]