View source for 6ioe


==Crystal structure of the CysR-CTLD2 fragment of human MR at basic pH (pH 8.5)==
<StructureSection load='6ioe' size='340' side='right'caption='[[6ioe]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ioe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IOE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ioe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ioe OCA], [https://pdbe.org/6ioe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ioe RCSB], [https://www.ebi.ac.uk/pdbsum/6ioe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ioe ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MRC1_HUMAN MRC1_HUMAN] Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
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== Publication Abstract from PubMed ==
Mannose receptor (MR, CD206) is an immune receptor highly expressed on macrophages and plays important roles in glycoprotein clearance, immune response and matrix turnover. Previous studies have shown that MR recognizes multiple ligands and recycles between cell surface and endosomes, and the conformation and ligand binding of MR are regulated by environmental pH. However, due to the lack of high-resolution details, the mechanisms of the pH-dependent properties of MR have not been fully understood. Here we investigate the pH-dependent conformational change of MR by solving a series of crystal structures of MR N-terminal fragments (CysR~CTLD2/3) at pH ranging from 4.0 to 8.5. The results show that the CTLD3 domain plays a critical role in regulating the conformational change of the N-terminal region of MR by forming interactions with the CTLD2 domain specifically at acidic pH. Moreover, the structural data also show the conformational changes of the 4-SO4-GalNAc binding pocket at the CysR domain, which might be relevant to the binding and release of the ligand. Overall, these results provide a model for the pH-dependent conformational change of the N-terminal region of MR that may help to understand its functional mechanism at molecular level.

Structural basis of the pH-dependent conformational change of the N-terminal region of human mannose receptor/CD206.,Hu Z, Wang Y, Cheng C, He Y J Struct Biol. 2019 Dec 1;208(3). pii: S1047-8477(19)30185-6. doi:, 10.1016/j.jsb.2019.09.001. Epub 2019 Sep 3. PMID:31491467<ref>PMID:31491467</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6ioe" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: He Y]]
[[Category: Hu Z]]