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==Cryo-EM structure of multidrug efflux pump MexAB-OprM (0 degree state)==
<SX load='6iok' size='340' side='right' viewer='molstar' caption='[[6iok]], [[Resolution|resolution]] 3.64&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6iok]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IOK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.64&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iok OCA], [https://pdbe.org/6iok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iok RCSB], [https://www.ebi.ac.uk/pdbsum/6iok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iok ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/MEXB_PSEAE MEXB_PSEAE] The inner membrane transporter component of the MexAB-OprM efflux system that confers multidrug resistance. Also functions as the major efflux pump for n-hexane and p-xylene efflux. Over-expression of the pump increases antibiotic and solvent efflux capacities. Implicated in the secretion of the siderophore pyoverdine.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref>   The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB subunit is a proton antiporter.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref> 
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== Publication Abstract from PubMed ==
In Pseudomonas aeruginosa, MexAB-OprM plays a central role in multidrug resistance by ejecting various drug compounds, which is one of the causes of serious nosocomial infections. Although the structures of the components of MexAB-OprM have been solved individually by X-ray crystallography, no structural information for fully assembled pumps from P. aeruginosa were previously available. In this study, we present the structure of wild-type MexAB-OprM in the presence or absence of drugs at near-atomic resolution. The structure reveals that OprM does not interact with MexB directly, and that it opens its periplasmic gate by forming a complex. Furthermore, we confirm the residues essential for complex formation and observed a movement of the drug entrance gate. Based on these results, we propose mechanisms for complex formation and drug efflux.

Structures of the wild-type MexAB-OprM tripartite pump reveal its complex formation and drug efflux mechanism.,Tsutsumi K, Yonehara R, Ishizaka-Ikeda E, Miyazaki N, Maeda S, Iwasaki K, Nakagawa A, Yamashita E Nat Commun. 2019 Apr 3;10(1):1520. doi: 10.1038/s41467-019-09463-9. PMID:30944318<ref>PMID:30944318</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6iok" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Nakagawa A]]
[[Category: Tsutsumi K]]
[[Category: Yamashita E]]
[[Category: Yonehara R]]