View source for 6ip9


==Crystal Structure of Lanthanum ion (La3+) bound bovine alpha-lactalbumin==
<StructureSection load='6ip9' size='340' side='right' caption='[[6ip9]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ip9]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5x84 5x84]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IP9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IP9 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ip9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ip9 OCA], [http://pdbe.org/6ip9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ip9 RCSB], [http://www.ebi.ac.uk/pdbsum/6ip9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ip9 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/LALBA_BOVIN LALBA_BOVIN]] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins. 
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== Publication Abstract from PubMed ==
Cancer remains one of the biggest threats to human society. There are massive demands for compounds to selectively kill cancerous cells. Earlier studies have shown that bovine alpha -lactalbumin made lethal to tumor cells (BAMLET) becomes cytotoxic against cancer cells in complex with oleic acid {Hoque, M. et. al., PLoSOne 8, e68390 (2013)}. In our study, we obtained bovine alpha-lactalbumin complexed with lanthanum ion (La(3+)-B-alpha-LA) and determined its high resolution crystal structure. The natural calcium binding site of bovine alpha-lactalbumin is replaced by lanthanum. The La(3+) complex formation by B-alpha-apo-LA was also supported by various biophysical methods. Interestingly, our complex, La(3+)-B-alpha-LA exhibits much greater anticancer activity against breast cancer cells as compared to the reported BAMLET-oleic acid complex. This study shows that La(3+)-B-alpha-LA complex is preferentially more toxic to MCF-7 cells as compared to KB (oral cancer) and HeLa (cervical) cells, while almost non-toxic to the healthy cells that we studied. Our data indicates that the cytotoxicity of La(3+)-B-alpha-LA against cancer cells is through apoptotic path way. The higher anticancer activity of La(3+)-B-alpha-LA is attributable to the requisite structural changes induced in the protein by La(3+) binding as supported by the crystal structure of the complex.

Cytotoxicity of apo bovine alpha-lactalbumin complexed with La(3+) on cancer cells supported by its high resolution crystal structure.,Yarramala DS, Prakash P, Ranade DS, Doshi S, Kulkarni PP, Bhaumik P, Rao CP Sci Rep. 2019 Feb 11;9(1):1780. doi: 10.1038/s41598-018-38024-1. PMID:30741951<ref>PMID:30741951</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ip9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bhaumik, P]]
[[Category: Prakash, P]]
[[Category: Rao, C P]]
[[Category: Yarramala, S D]]
[[Category: Bamlet]]
[[Category: Bovine alpha-lactalbumin]]
[[Category: Hamlet]]
[[Category: Lanthanum]]
[[Category: Metal binding protein]]