View source for 6itw


==Crystal structure of Atu4351 from Agrobacterium tumefaciens==
<StructureSection load='6itw' size='340' side='right'caption='[[6itw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6itw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ITW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ITW FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6itw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6itw OCA], [http://pdbe.org/6itw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6itw RCSB], [http://www.ebi.ac.uk/pdbsum/6itw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6itw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The type VI secretion system (T6SS) is a novel multiprotein needle-like apparatus that is distributed widely in Gram-negative bacteria. Bacteria harboring T6SSs inject various effectors into both eukaryotic and prokaryotic cells for interspecies competition or virulence-related processes. The toxicities of the effectors can be neutralized by their cognate immunity proteins. Tde1 (Atu4350)-Tdi1 (Atu4351) has recently been characterized as a T6SS effector-immunity pair in the soil bacterium Agrobacterium tumefaciens and the neutralization mechanism remains unknown. Here, the crystal structure of the immunity protein Tdi1 was determined at 2.40 A resolution by the single-wavelength anomalous dispersion method. Structural analysis suggested that it is composed of a GAD-like domain and an inserted DUF1851 domain, and both domains show low structural similarities to known structures. There is a positive groove mainly located in the GAD-like domain that may be associated with nucleotide binding. The structure provides a basis for further study of the positive groove as a potential active site.

Crystal structure of the type VI immunity protein Tdi1 (Atu4351) from Agrobacterium tumefaciens.,Shi L, Gao Z, Zhang T, Zhang H, Dong Y Acta Crystallogr F Struct Biol Commun. 2019 Mar 1;75(Pt 3):153-158. doi:, 10.1107/S2053230X19000815. Epub 2019 Feb 20. PMID:30839288<ref>PMID:30839288</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6itw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Dong, Y]]
[[Category: Gao, Z]]
[[Category: Shi, L]]
[[Category: Zhang, H]]
[[Category: Antitoxin]]
[[Category: Duf1851 domain]]
[[Category: Effector-immunity pair]]
[[Category: Gad-like domain]]
[[Category: Tdi1]]
[[Category: Type six secretion system]]