View source for 6ixi


==structure of Cd-bound periplasmic metal binding protein from candidatus liberibacter asiaticus==
<StructureSection load='6ixi' size='340' side='right'caption='[[6ixi]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ixi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Liberibacter_asiaticus_str._psy62 Candidatus Liberibacter asiaticus str. psy62]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IXI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ixi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ixi OCA], [https://pdbe.org/6ixi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ixi RCSB], [https://www.ebi.ac.uk/pdbsum/6ixi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ixi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C6XF58_LIBAP C6XF58_LIBAP] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Candidatus Liberibacter asiaticus (CLas), a phloem-limited unculturable Gram-negative bacterium, causes citrus greening disease. The proteome analysis of CLas showed the presence of a heavy metal permease and Co/Zn/Cd cation exporter system. However, there is no designated metal uptake protein specific for the heavy metal permease in CLas. One of the metal uptake proteins, designated as CLas-ZnuA2, in our previous studies, showed a lower metal-binding affinity for Mn2+ and Zn2+ and was postulated to bind and transport metals rather non-specifically. The present study focused on the characterization of the heavy metal binding properties of CLas-ZnuA2 using SPR, CD, DSC and crystallographic studies. The crystal structure analysis of Cd2+ bound CLas-ZnuA2 showed octahedral geometry for Cd2+ binding as compared to a non-preferred square-pyramidal geometry for Mn2+ and Zn2+ binding in earlier reported crystal structures. In SPR analysis, the binding affinities of 4.7 x 10-6 M, 7.2 x 10-6 M, 5.3 x 10-5 M and 4.3 x 10-5 M for Hg2+, Cd2+, Ba2+ and Co2+ respectively were higher as compared to earlier reported values for Mn2+ and Zn2+. Likewise, CD and DSC analysis showed relatively higher thermal stability for CLas-ZnuA2 on heavy metal binding. Taken together with the expression of the permease and exporter system for heavy metals, our results indicate that CLas-ZnuA2 may be involved in sequestering and transport of various transition divalent metals in environmentally stressed conditions.

Characterization of the heavy metal binding properties of periplasmic metal uptake protein CLas-ZnuA2.,Kumar P, Dalal V, Sharma N, Kokane S, Ghosh DK, Kumar P, Sharma AK Metallomics. 2020 Feb 26;12(2):280-289. doi: 10.1039/c9mt00200f. PMID:31853532<ref>PMID:31853532</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ixi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Candidatus Liberibacter asiaticus str. psy62]]
[[Category: Large Structures]]
[[Category: Dalal V]]
[[Category: Ghosh DK]]
[[Category: Kumar P]]
[[Category: Sharma AK]]
[[Category: Sharma N]]