View source for 6j35


==Crystal structure of ligand-free of PulA-G680L mutant from Klebsiella pneumoniae==
<StructureSection load='6j35' size='340' side='right'caption='[[6j35]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6j35]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J35 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.839&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j35 OCA], [https://pdbe.org/6j35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j35 RCSB], [https://www.ebi.ac.uk/pdbsum/6j35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j35 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/W9BQ28_KLEPN W9BQ28_KLEPN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Klebsiella pneumoniae pullulanase (KPP) belongs to glycoside hydrolase family 13 subfamily 13 (GH13_13) and is the only enzyme that is reported to perform an induced-fit motion of the active-site loop (residues 706-710). Comparison of pullulanase structures indicated that only KPP has Leu680 present behind the loop, in contrast to the glycine found in other GH13_13 members. Analysis of the structure and activity of recombinant pullulanase from K. pneumoniae ATCC 9621 (rKPP) and its mutant (rKPP-G680L) indicated that the side chain of residue 680 is important for the induced-fit motion of the loop 706-710 and alters the binding affinity of the substrate.

Relationship between the induced-fit loop and the activity of Klebsiella pneumoniae pullulanase.,Saka N, Malle D, Iwamoto H, Takahashi N, Mizutani K, Mikami B Acta Crystallogr D Struct Biol. 2019 Sep 1;75(Pt 9):792-803. doi: , 10.1107/S2059798319010660. Epub 2019 Aug 22. PMID:31478902<ref>PMID:31478902</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6j35" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Klebsiella pneumoniae]]
[[Category: Large Structures]]
[[Category: Iwamoto H]]
[[Category: Mikami B]]
[[Category: Mizutani K]]
[[Category: Saka N]]
[[Category: Takahashi N]]