View source for 6j38


==Crystal structure of CmiS2==
<StructureSection load='6j38' size='340' side='right'caption='[[6j38]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6j38]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._MJ635-86F5 Streptomyces sp. MJ635-86F5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J38 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j38 OCA], [https://pdbe.org/6j38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j38 RCSB], [https://www.ebi.ac.uk/pdbsum/6j38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j38 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/X5IYZ1_9ACTN X5IYZ1_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The flavin adenine dinucleotide-dependent oxidase CmiS2 catalyzes the oxidation of N-carboxymethyl-3-aminononanoic acid to produce a 3-aminononanoic acid starter unit for the biosynthesis of cremimycin, a macrolactam polyketide. Although the sequence of CmiS2 is similar with that of the well-characterized glycine oxidase ThiO, the chemical structure of the substrate of CmiS2 is different from that of ThiO substrate glycine. Here, we present the biochemical and structural characterization of CmiS2. Kinetic analysis revealed that CmiS2 has a strong preference for N-carboxymethyl-3-aminononanoic acid over other substrates such as N-carboxymethyl-3-aminobutanoic acid and glycine, suggesting that CmiS2 recognizes the nonanoic acid moiety of the substrate as well as the glycine moiety. We determined the crystal structure of CmiS2 in complex with a substrate analogue, namely, S-carboxymethyl-3-thiononanoic acid, which enabled the identification of key amino acid residues involved in substrate recognition. We discovered that Asn49, Arg243, and Arg334 interact with the carboxyl group of the nonanoic acid moiety, while Pro46, Leu52, and Ile335 recognize the alkyl chain of the nonanoic acid moiety via hydrophobic interaction. These residues are highly conserved in CmiS2 homologues involved in the biosynthesis of related macrolactam polyketides but are not conserved in glycine oxidases such as ThiO. These results suggest that CmiS2-type enzymes employ a distinct mechanism of substrate recognition for the synthesis of beta-amino acids.

Structural Analysis of the Glycine Oxidase Homologue CmiS2 Reveals a Unique Substrate Recognition Mechanism for Formation of a beta-Amino Acid Starter Unit in Cremimycin Biosynthesis.,Kawasaki D, Chisuga T, Miyanaga A, Kudo F, Eguchi T Biochemistry. 2019 Jun 4. doi: 10.1021/acs.biochem.9b00444. PMID:31154757<ref>PMID:31154757</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6j38" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces sp. MJ635-86F5]]
[[Category: Chisuga T]]
[[Category: Eguchi T]]
[[Category: Kawasaki D]]
[[Category: Kudo F]]
[[Category: Miyanaga A]]