View source for 6jau


==The complex structure of Pseudomonas aeruginosa MucA/MucB.==
<StructureSection load='6jau' size='340' side='right'caption='[[6jau]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jau]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JAU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.905&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jau OCA], [https://pdbe.org/6jau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jau RCSB], [https://www.ebi.ac.uk/pdbsum/6jau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jau ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MUCB_PSEAE MUCB_PSEAE] Negative regulator of the sigma factor AlgU. Plays a role in the differentiation of P.aeruginosa into the alginate-producing form. Inactivation of mucB causes conversion to mucoidy.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
MucA and MucB are critical negative modulators of sigma factor AlgU and regulate the mucoid conversion of Pseudomonas aeruginosa. Previous studies have revealed that lipid signals antagonize MucA-MucB binding. Here we report the crystal structure of MucB in complex with the periplasmic domain of MucA and polyethylene glycol (PEG), which unveiled an intermediate state preceding the MucA-MucB dissociation. Based on the biochemical experiments, the aliphatic side chain with a polar group was found to be of primary importance for inducing MucA cleavage. These results provide evidence that the hydrophobic cavity of MucB is a primary site for sensing lipid molecules and illustrates the detailed control of conformational switching within MucA-MucB in response to lipophilic effectors.

Molecular basis of the lipid-induced MucA-MucB dissociation in Pseudomonas aeruginosa.,Li T, He L, Li C, Kang M, Song Y, Zhu Y, Shen Y, Zhao N, Zhao C, Yang J, Huang Q, Mou X, Tong A, Yang J, Wang Z, Ji C, Li H, Tang H, Bao R Commun Biol. 2020 Aug 3;3(1):418. doi: 10.1038/s42003-020-01147-1. PMID:32747658<ref>PMID:32747658</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jau" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Bao R]]
[[Category: He LH]]
[[Category: Huang Q]]
[[Category: Li CC]]
[[Category: Li T]]
[[Category: Liu L]]
[[Category: Mu XY]]
[[Category: Peng CT]]
[[Category: Qin XF]]
[[Category: Shen YL]]
[[Category: Song YJ]]
[[Category: Xiao QJ]]
[[Category: Yang J]]
[[Category: Zhao C]]
[[Category: Zhao Nl]]
[[Category: Zhu YB]]