View source for 6jep


==Structure of a neutralizing antibody bound to the Zika envelope protein domain III==
<StructureSection load='6jep' size='340' side='right'caption='[[6jep]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jep]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Zika_virus Zika virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JEP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JEP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.316&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jep OCA], [https://pdbe.org/6jep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jep RCSB], [https://www.ebi.ac.uk/pdbsum/6jep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jep ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2Z2ESG6_ZIKV A0A2Z2ESG6_ZIKV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We previously reported a human monoclonal antibody, ZK2B10, capable of protection against Zika virus (ZIKV) infection and microcephaly in developing mouse embryos. Here, we report the structural features and mechanism of action of ZK2B10. The crystal structure at a resolution of 2.32 A revealed that the epitope is located on the lateral ridge of DIII of the envelope glycoprotein. Cryo-EM structure with mature ZIKV showed that the antibody binds to DIIIs around the icosahedral 2-fold, 3-fold, and 5-fold axes, a distinct feature compared to those reported for DIII-specific antibodies. The binding of ZK2B10 to ZIKV has no detectable effect on viral attachment to target cells or on conformational changes of the E glycoprotein in the acidic environment, suggesting that ZK2B10 functions at steps between the formation of the fusion intermediate and membrane fusion. These results provide structural and mechanistic insights into how ZK2B10 mediates protection against ZIKV infection.

Structural Basis for Neutralization and Protection by a Zika Virus-Specific Human Antibody.,Wang L, Wang R, Wang L, Ben H, Yu L, Gao F, Shi X, Yin C, Zhang F, Xiang Y, Zhang L Cell Rep. 2019 Mar 19;26(12):3360-3368.e5. doi: 10.1016/j.celrep.2019.02.062. PMID:30893607<ref>PMID:30893607</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jep" style="background-color:#fffaf0;"></div>

==See Also==
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Zika virus]]
[[Category: Ben HJ]]
[[Category: Gao F]]
[[Category: Shi XL]]
[[Category: Wang L]]
[[Category: Wang RK]]
[[Category: Xiang Y]]
[[Category: Yin CB]]
[[Category: Yu L]]
[[Category: Zhang FC]]
[[Category: Zhang LQ]]