View source for 6jfy


==GluK3 receptor trapped in Desensitized state==
<SX load='6jfy' size='340' side='right' viewer='molstar' caption='[[6jfy]], [[Resolution|resolution]] 7.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jfy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JFY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jfy OCA], [https://pdbe.org/6jfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jfy RCSB], [https://www.ebi.ac.uk/pdbsum/6jfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jfy ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/GRIK3_RAT GRIK3_RAT] Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA.<ref>PMID:21907808</ref> 
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== Publication Abstract from PubMed ==
GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission. For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy. While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation. Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties. The mutational analysis also identified putative N-glycan interacting residues. Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.

Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery.,Kumari J, Vinnakota R, Kumar J Sci Rep. 2019 Jul 16;9(1):10254. doi: 10.1038/s41598-019-46770-z. PMID:31311973<ref>PMID:31311973</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6jfy" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glutamate receptor 3D structures|Glutamate receptor 3D structures]]
== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Kumar J]]
[[Category: Kumari J]]