View source for 6jhy


==Crystal Structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein==
<StructureSection load='6jhy' size='340' side='right'caption='[[6jhy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jhy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dromedary_camel_coronavirus_HKU23 Dromedary camel coronavirus HKU23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JHY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jhy OCA], [https://pdbe.org/6jhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jhy RCSB], [https://www.ebi.ac.uk/pdbsum/6jhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jhy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/X2JHN8_9BETC X2JHN8_9BETC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The DcCoV UAE-HKU23 coronavirus is a newly-found betacoronavirus (betaCoV) that can infect human cells. The viral spike protein plays pivotal roles in mediating receptor-recognition and membrane-fusion, and is therefore a key factor involved in viral pathogenesis and inter-species transmission. Here we reported the structural and functional characterization of the spike N-terminal domain (NTD) from DcCoV UAE-HKU23 (HKU23-NTD). Via mucin-binding assays, we showed that HKU23-NTD is able to bind sugars. We further solved the structure of HKU23-NTD, performed structure-guided mutagenesis and successfully located the potential sugar-binding pockets in the structure. Furthermore, via comparison of available betaCoV NTD structures, we demonstrated that betaCoV NTDs contain a conserved beta-sandwich core, but exhibit variant folds in the peripheral elements located in the top-ceiling region and on the lateral side. While showing different compositions and structures, these peripheral elements are topologically equivalent beta-sandwich-core insertions, highlighting a divergent evolution process for betaCoVs to form different lineages.

Crystal structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein.,Cheng Y, He B, Yang J, Ye F, Lin S, Yang F, Chen Z, Chen Z, Cao Y, Lu G Virology. 2019 Jun 27;535:74-82. doi: 10.1016/j.virol.2019.06.015. PMID:31279241<ref>PMID:31279241</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jhy" style="background-color:#fffaf0;"></div>

==See Also==
*[[Sandbox 3001|Sandbox 3001]]
*[[Spike protein 3D structures|Spike protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dromedary camel coronavirus HKU23]]
[[Category: Large Structures]]
[[Category: Cheng Y]]
[[Category: Lu G]]
[[Category: Ye F]]