View source for 6jl1


==Crystal structure of VvPlpA G389D from Vibrio vulnificus==
<StructureSection load='6jl1' size='340' side='right'caption='[[6jl1]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JL1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jl1 OCA], [https://pdbe.org/6jl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jl1 RCSB], [https://www.ebi.ac.uk/pdbsum/6jl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jl1 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Phospholipases can disrupt host membranes and are important virulence factors in many pathogens. VvPlpA is a phospholipase A2 secreted by Vibrio vulnificus and essential for virulence. Its homologs, termed thermolabile hemolysins (TLHs), are widely distributed in vibrio bacteria but no structural information for this virulence factor class is available. Herein, we report the crystal structure of VvPlpA to 1.4 A resolution, revealing that VvPlpA contains an N-terminal domain of unknown function and a C-terminal phospholipase domain and that these two domains are packed closely together. The phospholipase domain adopts a typical SGNH hydrolase fold, containing the four conserved catalytic residues Ser, Gly, Asn, and His. Interestingly, the structure also disclosed that the phospholipase domain accommodates a chloride ion near the catalytic His residue. The chloride is five-coordinated in a distorted bipyramid geometry, accepting hydrogen bonds from a water molecule and the amino groups of surrounding residues. This chloride substitutes the most common Asp/Glu residue and forms an unusual Ser-His-chloride catalytic triad in VvPlpA. The chloride may orient the catalytic His and stabilize the charge on its imidazole ring during catalysis. Indeed, VvPlpA activity depended on chloride concentration, confirming the important role of chloride in catalysis. The VvPlpA structure also revealed a large hydrophobic substrate-binding pocket that is capable of accommodating a long-chain acyl group. Our results provide the first structure of the TLH family and uncover an unusual Ser-His-chloride catalytic triad, expanding our knowledge on the biological role of chloride.

Structural analysis of a vibrio phospholipase reveals an unusual Ser-His-chloride catalytic triad.,Wan Y, Liu C, Ma Q J Biol Chem. 2019 May 9. pii: RA119.008280. doi: 10.1074/jbc.RA119.008280. PMID:31073025<ref>PMID:31073025</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6jl1" style="background-color:#fffaf0;"></div>

==See Also==
*[[Hemolysin 3D structures|Hemolysin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio vulnificus]]
[[Category: Liu C]]
[[Category: Ma Q]]
[[Category: Wan Y]]