View source for 6jl3


==Crystal Structure of the UBL domain of Plasmodium Falciparum Dsk2==
<StructureSection load='6jl3' size='340' side='right'caption='[[6jl3]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jl3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JL3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JL3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.303&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jl3 OCA], [https://pdbe.org/6jl3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jl3 RCSB], [https://www.ebi.ac.uk/pdbsum/6jl3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jl3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8IIM8_PLAF7 Q8IIM8_PLAF7] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
One of the pathways by which proteins are targeted for degradation by the proteasome involve transport by shuttle proteins to proteasomal receptors. The malaria parasite Plasmodium falciparum has recently been found to possess a similar pathway, with the shuttle protein PfDsk2 being the major player. In this study, we have demonstrated how PfDsk2 and its recognition by proteasomal receptors differ from the mammalian system. Our crystal structure of unbound PfDsk2 UBL domain at 1.30A revealed an additional 310-helix compared to the human homolog, as well as a few significant differences in its putative binding interface with the proteasome receptors, PfRpn10 and PfRpn13. Moreover, the non-binding face of UBL showed a reversal of surface charge compared to HsDsk2 shuttle protein, instead resembling HOIL-like E3 ligase UBL domain. The affinity of the interaction with the proteasomal receptors remained similar to the human system, and dissociation constants of the same order of magnitude. On the other hand, we have found evidence of a novel interaction between PfRpn13(DEUBAD) with the PfDsk2(UBL) suggesting that PfDsk2 may work in cooperation with deubiquitinating enzymes for proofreading ubiquitinated substrates. Our study provides the first molecular look at shuttle proteins in Apicomplexan parasites and hints at how their interaction landscape might be broader than what we may expect.

The recognition of proteasomal receptors by Plasmodium falciparum DSK2.,Gupta I, Khan S Mol Biochem Parasitol. 2020 Feb 11;236:111266. doi:, 10.1016/j.molbiopara.2020.111266. PMID:32057831<ref>PMID:32057831</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jl3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Plasmodium falciparum 3D7]]
[[Category: Gupta I]]
[[Category: Khan S]]