View source for 6jlh


==Structure of SCGN in complex with a Snap25 peptide==
<StructureSection load='6jlh' size='340' side='right'caption='[[6jlh]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jlh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JLH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jlh OCA], [https://pdbe.org/6jlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jlh RCSB], [https://www.ebi.ac.uk/pdbsum/6jlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jlh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SEGN_DANRE SEGN_DANRE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Secretagogin (SCGN) is a hexa-EF-hand protein that is highly expressed in the pancreas, brain, and gastrointestinal tract. SCGN is known to modulate regulated exocytosis in multiple cell lines and tissues; however, its exact functions and underlying mechanisms remain unclear. Here, we report that SCGN interacts with the plasma membrane SNARE SNAP-25, but not the assembled SNARE complex, in a Ca(2+)-dependent manner. The crystal structure of SCGN in complex with a SNAP-25 fragment reveals that SNAP-25 adopts a helical structure and binds to EF-hands 5 and 6 of SCGN. SCGN strongly inhibits SNARE-mediated vesicle fusion in vitro by binding to SNAP-25. SCGN promotes the plasma membrane localization of SNAP-25, but not Syntaxin-1a, in SCGN-expressing cells. Finally, SCGN controls neuronal growth and brain development in zebrafish, likely via interacting with SNAP-25 or its close homolog, SNAP-23. Our results thus provide insights into the regulation of SNAREs and suggest that aberrant synapse functions underlie multiple neurological disorders caused by SCGN deficiency.

Structural and mechanistic insights into secretagogin-mediated exocytosis.,Qin J, Liu Q, Liu Z, Pan YZ, Sifuentes-Dominguez L, Stepien KP, Wang Y, Tu Y, Tan S, Wang Y, Sun Q, Mo X, Rizo J, Burstein E, Jia D Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6559-6570. doi:, 10.1073/pnas.1919698117. Epub 2020 Mar 10. PMID:32156735<ref>PMID:32156735</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jlh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Danio rerio]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Jia D]]
[[Category: Qin J]]
[[Category: Sun Q]]