View source for 6jxf


==Photoswitchable fluorescent protein Gamillus, off-state (pH7.0)==
<StructureSection load='6jxf' size='340' side='right'caption='[[6jxf]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jxf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Olindias Olindias]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JXF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=QYG:2-[(4~{E})-2-[(1~{S})-1,4-bis(azanyl)-4-oxidanylidene-butyl]-4-[(4-hydroxyphenyl)methylidene]-5-oxidanylidene-imidazol-1-yl]ethanoic+acid'>QYG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jxf OCA], [https://pdbe.org/6jxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jxf RCSB], [https://www.ebi.ac.uk/pdbsum/6jxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jxf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2Z5X7U3_9CNID A0A2Z5X7U3_9CNID] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Reversibly switchable fluorescent proteins (RSFPs) are crucial tags for super-resolution observation of protein localization and dynamics inside living cells. However, due to the high fluorescence pKa ( approximately 5-6) of most RSFPs, their usage in acidic conditions (pH 4.5-6.0) has been limited. Here, we investigated a new photochromic mechanism in Gamillus, a recently developed green fluorescent protein with acid tolerance. Gamillus exhibits negative switching with especially high contrast in acidic conditions, and its off switching is caused by trans-to-cis isomerization of the chromophore hydroxyphenyl ring that accompanies protonation. Through a combination of rational design and saturation mutagenesis, we developed two variants with enhanced switching contrasts and off-switching speeds, designated rsGamillus-S and rsGamillus-F, respectively. The fluorescence intensity, off-switching speed, and switching contrast of the rsGamillus variants are only slightly affected by changes in pH between 4.5 and 7.5. Exploiting these properties, we succeeded in high-contrast super-resolution imaging of cellular architectures in acidic conditions.

Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions.,Shinoda H, Lu K, Nakashima R, Wazawa T, Noguchi K, Matsuda T, Nagai T Cell Chem Biol. 2019 Oct 17;26(10):1469-1479.e6. doi:, 10.1016/j.chembiol.2019.07.012. Epub 2019 Aug 15. PMID:31422907<ref>PMID:31422907</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jxf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Olindias]]
[[Category: Matsuda T]]
[[Category: Nagai T]]
[[Category: Nakashima R]]
[[Category: Sakurai K]]
[[Category: Shinoda H]]