View source for 6jxh


==K+-bound E2-MgF state of the gastric proton pump (Tyr799Trp)==
<StructureSection load='6jxh' size='340' side='right'caption='[[6jxh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jxh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JXH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CE1:O-DODECANYL+OCTAETHYLENE+GLYCOL'>CE1</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MF4:TETRAFLUOROMAGNESATE(2-)'>MF4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCW:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PCW</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jxh OCA], [https://pdbe.org/6jxh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jxh RCSB], [https://www.ebi.ac.uk/pdbsum/6jxh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jxh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The gastric proton pump (H(+),K(+)-ATPase), a P-type ATPase responsible for gastric acidification, mediates electro-neutral exchange of H(+) and K(+) coupled with ATP hydrolysis, but with an as yet undetermined transport stoichiometry. Here we show crystal structures at a resolution of 2.5 A of the pump in the E2-P transition state, in which the counter-transporting cation is occluded. We found a single K(+) bound to the cation-binding site of the H(+),K(+)-ATPase, indicating an exchange of 1H(+)/1K(+) per hydrolysis of one ATP molecule. This fulfills the energy requirement for the generation of a six pH unit gradient across the membrane. The structural basis of K(+) recognition is resolved and supported by molecular dynamics simulations, establishing how the H(+),K(+)-ATPase overcomes the energetic challenge to generate an H(+) gradient of more than a million-fold-one of the highest cation gradients known in mammalian tissue-across the membrane.

A single K(+)-binding site in the crystal structure of the gastric proton pump.,Yamamoto K, Dubey V, Irie K, Nakanishi H, Khandelia H, Fujiyoshi Y, Abe K Elife. 2019 Aug 22;8. pii: 47701. doi: 10.7554/eLife.47701. PMID:31436534<ref>PMID:31436534</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jxh" style="background-color:#fffaf0;"></div>

==See Also==
*[[ATPase 3D structures|ATPase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Abe K]]
[[Category: Irie K]]