View source for 6jxm


==Crystal Structure of phi29 pRNA domain II==
<StructureSection load='6jxm' size='340' side='right'caption='[[6jxm]], [[Resolution|resolution]] 3.32&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jxm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JXM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.32&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jxm OCA], [https://pdbe.org/6jxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jxm RCSB], [https://www.ebi.ac.uk/pdbsum/6jxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jxm ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Packaging of phage phi29 genome requires the ATPase gp16 and prohead RNA (pRNA). The highly conserved pRNA forms the interface between the connector complex and gp16. Understanding how pRNA interacts with gp16 under packaging conditions can shed light on the molecular mechanism of the packaging motor. Here, we present 3D models of the pRNA-gp16 complex and its conformation change in response to ATP or ADP binding. Using a combination of crystallography, small angle X-ray scattering and chemical probing, we find that the pRNA and gp16 forms a 'Z'-shaped complex, with gp16 specifically binds to pRNA domain II. The whole complex closes in the presence of ATP, and pRNA domain II rotates open as ATP hydrolyzes, before resetting after ADP is released. Our results suggest that pRNA domain II actively participates in the packaging process.

ATP/ADP modulates gp16-pRNA conformational change in the Phi29 DNA packaging motor.,Cai R, Price IR, Ding F, Wu F, Chen T, Zhang Y, Liu G, Jardine PJ, Lu C, Ke A Nucleic Acids Res. 2019 Aug 9. pii: 5545366. doi: 10.1093/nar/gkz692. PMID:31396619<ref>PMID:31396619</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jxm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus virus phi29]]
[[Category: Large Structures]]
[[Category: Cai R]]
[[Category: Chen T]]
[[Category: Ding F]]
[[Category: Jardine PJ]]
[[Category: Ke A]]
[[Category: Liu G]]
[[Category: Lu C]]
[[Category: Price IR]]
[[Category: Wu F]]
[[Category: Zhang Y]]