View source for 6jyi


==Crystal structure of the PadR-like transcriptional regulator BC1756 from Bacillus cereus==
<StructureSection load='6jyi' size='340' side='right'caption='[[6jyi]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jyi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_14579 Bacillus cereus ATCC 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JYI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jyi OCA], [https://pdbe.org/6jyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jyi RCSB], [https://www.ebi.ac.uk/pdbsum/6jyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jyi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q81F45_BACCR Q81F45_BACCR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Transcription factors that belong to the PadR family play an essential role in the transcriptional regulation of diverse biological processes by recognizing their cognate palindromic DNA sequences. Bacillus cereus harbors a gene that encodes a PadR-like protein (bcPLP; BC1756). bcPLP has not been structurally characterized, and it remains unelucidated how bcPLP interacts with a specific DNA sequence to function as a transcription factor. To provide structural insights into DNA recognition by bcPLP, we performed a structural study and a DNA-binding analysis of bcPLP. The crystal structure of bcPLP was determined at 1.92A resolution. bcPLP consists of two domains, an N-terminal domain (NTD) and a C-terminal domain (CTD), and forms a homodimer mainly using the CTD. In the structure, bcPLP contains a highly positively charged elongated patch in the NTD that serves as a putative DNA-binding site. Indeed, an electrophoresis mobility shift assay and a fluorescence polarization assay showed that bcPLP specifically recognizes a palindromic DNA sequence upstream of the bcPLP-encoding region. Moreover, based on our mutagenesis and modeling studies, we demonstrate that bcPLP interacts with dsDNA primarily using the Y19, Y41, P64, and K66 residues in the NTD.

Structural and DNA-binding studies of the PadR-like transcriptional regulator BC1756 from Bacillus cereus.,Kim TH, Park SC, Lee KC, Song WS, Yoon SI Biochem Biophys Res Commun. 2019 Jun 6. pii: S0006-291X(19)31037-X. doi:, 10.1016/j.bbrc.2019.05.141. PMID:31178139<ref>PMID:31178139</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jyi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus cereus ATCC 14579]]
[[Category: Large Structures]]
[[Category: Kim TH]]
[[Category: Lee KC]]
[[Category: Park SC]]
[[Category: Song WS]]
[[Category: Yoon SI]]