View source for 6jyv


==Structure of an isopenicillin N synthase from Pseudomonas aeruginosa PAO1==
<StructureSection load='6jyv' size='340' side='right'caption='[[6jyv]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6jyv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JYV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.651&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jyv OCA], [https://pdbe.org/6jyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jyv RCSB], [https://www.ebi.ac.uk/pdbsum/6jyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jyv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HWJ0_PSEAE Q9HWJ0_PSEAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Isopenicillin N synthase (IPNS) is a nonheme-Fe(2+)-dependent enzyme that mediates a key step in penicillin biosynthesis. It catalyses the conversion of the tripeptide delta-(l-alpha-aminoadipoyl)-l-cysteine-d-valine (ACV) to isopenicillin N, which is a key precursor to beta-lactam antibiotics. The pa4191 gene in Pseudomonas aeruginosa PAO1 has provisionally been annotated as a member of the IPNS family. In this work, we report the crystal structure of PA4191 from P. aeruginosa (PaIPNS hereafter). The 1.65A resolution PaIPNS structure forms a jelly roll fold and is confirmed to be a member of the IPNS family based on structural homology. A metal centre within the jelly roll consists of the strictly conserved His201, Asp203 and His257 residues. MicroScale Thermophoresis binding analysis confirms that PaIPNS is a metal-binding protein with a strong preference for iron, but that it does not bind the tripeptide ACV. Structural comparison of PaIPNS with a previously reported IPNS-ACV complex structure reveals a restricted binding pocket that is unable to accommodate ACV.

Structural characterization of an isopenicillin N synthase family oxygenase from Pseudomonas aeruginosa PAO1.,Zhang H, Che S, Wang R, Liu R, Zhang Q, Bartlam M Biochem Biophys Res Commun. 2019 May 13. pii: S0006-291X(19)30932-5. doi:, 10.1016/j.bbrc.2019.05.062. PMID:31097228<ref>PMID:31097228</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6jyv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Bartlam M]]
[[Category: Che S]]
[[Category: Hao Z]]
[[Category: Liu R]]
[[Category: Wang R]]
[[Category: Zhang Q]]