View source for 6k6b


==Application of anti-helix antibodies in protein structure determination (8496-3LRH)==
<StructureSection load='6k6b' size='340' side='right'caption='[[6k6b]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6k6b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K6B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k6b OCA], [https://pdbe.org/6k6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k6b RCSB], [https://www.ebi.ac.uk/pdbsum/6k6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k6b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPA_STAA8 SPA_STAA8] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Antibodies are indispensable tools in protein engineering and structural biology. Antibodies suitable for structural studies should recognize the 3-dimensional (3D) conformations of target proteins. Generating such antibodies and characterizing their complexes with antigens take a significant amount of time and effort. Here, we show that we can expand the application of well-characterized antibodies by "transplanting" the epitopes that they recognize to proteins with completely different structures and sequences. Previously, several antibodies have been shown to recognize the alpha-helical conformation of antigenic peptides. We demonstrate that these antibodies can be made to bind to a variety of unrelated "off-target" proteins by modifying amino acids in the preexisting alpha helices of such proteins. Using X-ray crystallography, we determined the structures of the engineered protein-antibody complexes. All of the antibodies bound to the epitope-transplanted proteins, forming accurately predictable structures. Furthermore, we showed that binding of these antihelix antibodies to the engineered target proteins can modulate their catalytic activities by trapping them in selected functional states. Our method is simple and efficient, and it will have applications in protein X-ray crystallography, electron microscopy, and nanotechnology.

Application of antihelix antibodies in protein structure determination.,Kim JW, Kim S, Lee H, Cho G, Kim SC, Lee H, Jin MS, Lee JO Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1910080116. doi:, 10.1073/pnas.1910080116. PMID:31371498<ref>PMID:31371498</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6k6b" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Cho GY]]
[[Category: Jin MS]]
[[Category: Kim JW]]
[[Category: Kim S]]
[[Category: Lee H]]
[[Category: Lee JO]]