View source for 6kaw


==Crystal structure of CghA==
<StructureSection load='6kaw' size='340' side='right'caption='[[6kaw]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kaw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_globosum_CBS_148.51 Chaetomium globosum CBS 148.51]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KAW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kaw OCA], [https://pdbe.org/6kaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kaw RCSB], [https://www.ebi.ac.uk/pdbsum/6kaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kaw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CGHA_CHAGB CGHA_CHAGB] Diels-Alderase; part of the gene cluster that mediates the biosynthesis of the tetramic acid Sch210972, a potential anti-HIV fungal natural product that contains a decalin core (PubMed:26360642). The PKS module of cghG together with the enoylreductase cghC catalyze the formation of the polyketide unit which is then conjugated to 4-hydroxyl-4-methyl glutamate (HMG) by the condensation domain of the cghG NRPS module (PubMed:26360642). One unique structural feature of Sch210972 is the tetramic acid motif proposed to be derived from the non-proteinogenic amino acid HMG, by a Dieckmann-type condensation catalyzed by the reductase domain of cghG (PubMed:26360642). The aldolase cghB catalyzes the aldol condensation of 2 molecules of pyruvic acid to yield the intermediate 4-hydroxyl-4-methyl-2-oxoglutarate (HMOG), which can then be stereoselectively transaminated by an unidentified enzyme to form HMG (PubMed:26360642). The Diels-Alderase cghA then uses the Dieckmann product released by cghG as substrate and catalyzes the Diels-Alder cycloaddition to form the decalin ring of Sch210972 (PubMed:26360642). CghA also suppresses the nonenzymatic formation of the alternative stereoisomer (PubMed:26360642).<ref>PMID:26360642</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Chaetomium globosum CBS 148 51]]
[[Category: Large Structures]]
[[Category: Hara K]]
[[Category: Hashimoto H]]
[[Category: Sato M]]
[[Category: Watanabe K]]
[[Category: Yokoyama M]]